Yamaguchi University

Jun Asakawa

Rinji Akada

Hisashi Hoshida

Babiker Mohamed Ahmed Abdel-Banat

An object to be solved by the present invention is to provide, for example, a method for producing atransformant by a method for conveniently and efficiently connecting the ends of DNA fragments without using specific restriction enzymes or their recognition sequences, and a method for producing a useful substance using the transformant. The present inventors have found, as means to solve the object, a method comprising introducing two or more linear double-stranded DNA fragments free from aautonomously replicating sequence intoand selecting a transformant comprising a desired DNA ligation product with marker gene expression by the desired DNA ligation product as an index, or comprising introducing a linear double-stranded DNA fragment comprising aautonomously replicating sequence, alone or in combination with one or more linear double-stranded DNA fragment(s) different therefrom intoand selecting a transformant comprising a desired circular DNA ligation product with marker gene expression by the desired circular DNA ligation product as an index.

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Ube, Japan

Jun Asakawa