Seikagaku Kogyo Kabushiki Kaisha (seikagaku Corporation)

Seikagaku Kogyo Co. Ltd.

Seikagaku Corporation

Seikagaku Kogyo Kabushiki Kaisha

Jun Aketagawa

Shigenori Tanaka

Hiroshi Tamura

Yuko Shibata

Toshio Oda

Shoji Takahashi

Makoto Ohki

Maki Aizawa

To provide means for reducing reaction interference observed when AT III is subjected totest, whereby thetest of antithrombin III can be carried out at high accuracy. Reaction interference observed when AT III is subjected totest can be reduced by subjecting AT III to a protein inactivation treatment in the co-presence of a divalent metal salt.

Pre-treatment agent and pre-treatment method for antithrombin III to be subjected to limulus test

A pretreatment agent for a sample to be subjected toassay comprising an alkali metal sulfate and/or an alkaline earth metal sulfate wherein the sulfate(s) has a final concentration of 20 mM or more when the sulfate(s) is allowed to contact with the sample, or an alkali metal halide and/or an alkaline earth metal halide wherein the halide(s) has a final concentration of from 0.4 M to 1.2 M or less when the halide(s) is allowed to contact with the sample, or a kit forassay reagent comprising thereof as a composing article.

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An endotoxin stabilizing agent is provided, which is useful in order to maintain the endotoxin activity of a specimen or a reference standard in a stable state for a prolonged period of time and to prepare an endotoxin reference standard having a reduced intervial variation, being stable for a long time in the form of, in particular, a solution and withstanding repeated use. An endotoxin composition comprising the above-mentioned endotoxin stabilizing agent and endotoxin and a method for assaying endotoxin by using the same are also provided. The endotoxin stabilizing agent comprises: (1) at least one substance selected from the group consisting of aminoalcohols, polyhydric alcohols, nonionic surfactants, polysucroses and chelating agents; (2) at least aminoalcohol and polyhydric alcohol; (3) with the polyhydric alcohol being preferably glycerol or its derivative; (4) polyethylene glycol as an effective ingredient in addition to the components (1) to (3); or (5) at least polysucrose, and an alkaline earth metal salt and/or polyethylene glycol as an effective ingredient. The endotoxin composition comprises the above-identified endotoxin stabilizing agent. The method for assaying endotoxin comprises adding the above-described endotoxin stabilizing agent to a specimen to thereby stabilize the activity of endotoxin in the specimen.

Endotoxin stabilizing agent, endotoxin composition and method for

This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

Horseshoe crab amebocyte lysate factor G activation inhibitor

The invention stabilizes single helix conformation (1.fwdarw.3)-.beta.-D-glucans by intramolecular crosslinking and provides (1.fwdarw.3)-.beta.-D-glucans showing stable biological activities. Thus, intramolecularly crosslinked (1.fwdarw.3)-.beta.-D-glucans having a stable single helix conformation are produced by introducing a functional group into hydroxyl groups of (1.fwdarw.3)-.beta.-D-glucans, then causing the thus-obtained functional glucans to take a single helix conformation, and subjecting these, either as such or after binding them to a receptor therefor, to intramolecular crosslinking between the functional groups introduced, if necessary followed by releasing the receptor therefrom.

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Tokyo, Japan

Jun Aketagawa