Life Technologies Corporation

Julio Mulero

Robert Lagace

Robert L Green

Lori Hennessy

Dennis Wang

Michael Malicdem

Christina Bormann Chung

Steven Menchen

Chien-Wei Chang

Chang Zhong

Novel Y-STR multiplex analysis designs, primer design, allelic ladders, methods of use and kits are disclosed, including the use of primer sets designed to provide amplicons for at least 11 Y-STR loci having a base pair size of less than about 220 bp, as well as the use of primer sets designed to provide amplicons for at least 22 Y-STR loci including at least 5 rapidly mutating loci.

Multiplex Y-STR analysis

Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

SE33 mutations impacting genotype concordance

Disclosed herein are mesylate containing compositions, methods of use of such compositions and kits of components encompassing such compositions for improving the amplification of nucleic acids, especially in the presence of enzymatic inhibitors. These find particular use in forensic and environmental analyses. The instant disclosure provides new compositions, methods and kits of components for overcoming PCR inhibition. In some embodiments disclosed herein is a PCR master mix, the PCR master mix encompassing a mesylate.

Mesylate based master mix

Disclosed are methods for human identification utilizing newly discovered single nucleotide polymorphisms (SNPs) within CODIS loci which can cause allelic dropout. Also disclosed are kits useful in human identification.

Human single nucleotide polymorphisms

Disclosed are methods for human identification utilizing newly discovered single nucleotide polymorphisms (SNPs) within CODIS loci D13S317, TH01, vWA, D12S391 and D6S1043, which can cause allelic dropout. Also disclosed are kits useful in human identification.

Human single nucleotide polymorphisms within CODIS loci D13S317, TH01, vWA, D12S391, and D6S1043

Disclosed are methods for gender determination in the intron 1 region of the amelogenin locus and a newly discovered single nucleotide polymorphism (SNP) within the X chromosome of the amelogenin locus which can cause allelic dropout. Also disclosed are kits useful in gender determination.

Amelogenin SNP on chromosome X

Disclosed are rare short tandem repeat (STR) alleles within the D10S1248 and D12S391 loci in humans. Provided are representative allelic ladders for each locus, methods and assays using these alleles and kits containing allelic ladders comprising these alleles for accurate genotyping and identification of a wide range of individuals.

Growing community of inventors

Sunnyvale, CA, United States of America

Julio Mulero