Becton Dickinson and Company Limited

Cellular Research, Inc.

Julie Wilhelmy

Glenn K Fu

Stephen P A Fodor

Christina Fan

Eleen Shum

Geoffrey Richard Facer

Stephen Pa Fodor

Stephen PA Fodor

Maria Simbirsky

The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

Massively parallel single cell analysis

Methods, kits and systems are disclosed for analyzing one or more molecules in a sample. Analyzing the one or more molecules may comprise quantitation of the one or more molecules. Individual molecules may quantitated by PCR, arrays, beads, emulsions, droplets, or sequencing. Quantitation of individual molecules may further comprise stochastic labeling of the one or more molecules with a plurality of oligonucleotide tags to produce one or more stochastically labeled molecules. The methods may further comprise amplifying, sequencing, detecting, and/or quantifying the stochastically labeled molecules. The molecules may be DNA, RNA and/or proteins.

Compositions and kits for molecular counting

Provided herein are methods, compositions, systems, devices, and kits for quantification of RNA, and determination of efficiency of reverse transcription of RNA to cDNA.

Methods for RNA quantification

Massively Parallel single cell analysis

Growing community of inventors

Santa Cruz, CA, United States of America

Julie Wilhelmy