The Secretary of State for Defence

Julie Miller

Ethel Diane Williamson

Nicola Jane Walker

Leslie William James Baillie

Helen Claire Flick-Smith

Richard William Titball

Helen Lisa Bullifent

Paula Thomson Holden

Andrew William Topping

James Edward Eyles

Emma Louise Waters

Leslie William Baillie

Topping Andrew William

Leslie William Jones Baillie

An immunogenic reagent which produces an immune response which is protective against, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) ofor variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.

Expression system

Spore coat-associated proteins from members ofgenera, and in particular spore-coat associated protein N (CotN), have utilization as adjuvants in vaccine formulations. The vaccine formulations most likely contain a virulence factor of bacterial origin, which in the case ofgenera is the protective antigen.

Vaccine formulation

An immunogenic reagent which produces an immune response which is protective againstsaid reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) ofor variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression fromHigh yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.

A recombinant microorganism comprises an asporogenicstrain in which a gene encoding a protease enzyme has been downregulated or inactivated. In particular sigma factorspoIIAC is inactivated such that the strain is asporogenic. These strains are particularly useful as expression vehicles for proteins such as protective antigen (PA) of

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Salisbury, United Kingdom

Julie Miller