Arkion Life Sciences, LLC

Bio-Technical Resources, a Division of Arkion Life Sciences, LLC

Dcv, Inc.

Other

Dcv Inc. Doing Business as Bio-Technical Resourses

Julie Maurina-Brunker

James R Millis

Thomas W McMullin

Gabriel G Saucy

John Anthony Hyatt

Alan D Grund

Thomas W Mcmullin

A recombinant yeast that makes an isoprenoid compound is provided. The yeast comprises an endogenous mevalonate pathway comprising (i) an enzyme that converts acetyl-CoA to acetoacetyl-CoA, (ii) an enzyme that converts acetoacetyl-CoA to hydroxymethylglutaryl-CoA, (iii) an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, (iv) an enzyme that converts mevalonic acid to phosphomevalonic acid, (v) an enzyme that converts phosphomevalonic acid to phosphomevalonate, and (vi) an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. The yeast further comprises heterologous nucleic acid sequences encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, an enzyme that converts mevalonic acid to phosphomevalonic acid, an enzyme that converts phosphomevalonic acid to phosphomevalonate, and an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. Carbon flow through the mevalonate pathway is increased in the recombinant yeast compared to a yeast that does not comprise the heterologous nucleic acid molecule encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid.

Production of isoprenoids

The invention provides a biological method of producing isoprenoids.

The invention provides a biological method of producing farnesol or geranylgeraniol.

Production of farnesol and geranylgeraniol

The invention provides a biological method of producing geranylgeraniol.

Method of producing geranylgeraniol

The invention provides a method of producing &agr;-tocopherol and &agr;-tocopheryl esters. The method comprises using a biological system to produce farnesol or geranylgeraniol. Then, the farnesol or geranylgernaiol is chemically converted into &agr;-tocopherol or an &agr;-tocopheryl ester.

Method of vitamin production

The present invention relates to novel arylsulfotransferases. In particular, the present invention relates to novel arylsulfotransferas from Clostridium and from

Growing community of inventors

Appleton, WI, United States of America

Julie Maurina-Brunker