Swift Biosciences, Inc.

Integrated Dna Technologies, Inc.

Julie Laliberte

Vladimir L Makarov

The present disclosure describes a method of adapter ligation to the ends of fragmented double-stranded DNA molecules.

Enhanced adaptor ligation

Methods for performing multiplex PCR-based enrichment of a target substrate are provided. Systems and methods for generating a sequencing library are also provided.

Methods for multiplex PCR

Compositions and methods for enhanced adapter ligation are provided. The compositions and methods can be useful for next generation sequencing (NGS) library preparation and can result in higher yields in an NGS library.

Compositions and methods for enhanced adapter ligation

Methods for the preparation of PCR reaction mixtures and for performing multiplex PCR amplification which limit the production of non-target amplicons are provided. Production of non-target amplicons is limited because the non-target amplicons have complementary 5' and 3&#x2032; ends as a result of the target-specific primer designs such that the non-target amplicons can form stable secondary structure during the annealing step.

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Ypsilanti, MI, United States of America

Julie Laliberte