Becton Dickinson and Company Limited

Arbor Vita Corporation

Julie L Rosales

Adrien P Malick

John D Mantlo

Patrick Shawn Beaty

Susan M Kircher

William B Brasso

Ben Turng

Liping Feng

Vanda White

Dyan Luper

Michael A Brasch

Kerry Lynn Smith

Virginia M Crews

Suneil Hosmane

James Y Zhou

Donald Ray Callihan

Curtis M Gosnell

David Polley

Jeffery H Bruton

Carrie S Ferguson

Jeff H Bruton

Robert J Beadenkopf

John P Douglass

Song Shi

Gretta Campbell

Jefferey H Bruton

Donald R Callihan

Shawn Beaty

A system and method with increased sensitivity to microorganism growth. The system includes signal processing electronic circuit connected to a consumable or vessel through two or more electrodes that fully penetrate the vessel and are in contact with the fluid contents. The electronic circuit is configured to detect a component of the total impedance of the sample, specifically the “out-of-phase” or imaginary reactance component, which has a sensitive response to organism growth in a frequency-dependent manner. The system detects changes in both the composition of charged molecules in the liquid matrix and the number of microorganisms based on monitoring the sample for change in this parameter. This results in a 5-70% reduction in time-to-detection (TTD). The system and method detect organisms in a plurality of vessel shapes, volumes, and matrix (or media) formats. The electrodes are fully immersed in a continuous body of liquid sample. The distance between electrodes may be adjusted or tuned to fit the needs of the vessel. The voltage inputs can also be adjusted to allow proper detection of the contents within the vessel.

Reactance and capacitance sensing platform for detecting microorganisms

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Formulations and process for isolating viable microorganisms from positive blood cultures

A system and method with increased sensitivity to microorganism growth. The system includes signal processing electronic circuit connected to a consumable or vessel through two or more electrodes that fully penetrate the vessel and are in contact with the fluid contents. The electronic circuit is configured to detect a component of the total impedance of the sample, specifically the 'out-of-phase' or imaginary reactance component, which has a sensitive response to organism growth in a frequency-dependent manner. The system detects changes in both the composition of charged molecules in the liquid matrix and the number of microorganisms based on monitoring the sample for change in this parameter. This results in a 5-70% reduction in time-to-detection (TTD). The system and method detect organisms in a plurality of vessel shapes, volumes, and matrix (or media) formats. The electrodes are fully immersed in a continuous body of liquid sample. The distance between electrodes may be adjusted or tuned to fit the needs of the vessel. The voltage inputs can also be adjusted to allow proper detection of the contents within the vessel.

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.

Method of efficient extraction of protein from cells

Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture ('PBC') samples for use in downstream analyses such as identification and antimicrobial susceptibility testing ('AST'). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of

Method for direct and rapid identification of microorganisms and antimicrobial susceptibility testing from positive blood cultures

Growing community of inventors

Randallstown, MD, United States of America

Julie L Rosales