Research Institute for Genetic and Human Therapy (r.i.g.h.t.)

Genetic Immunity, LLC

Other

The United States of America, As Represented by the Secretary, Department of Health and Human Services

R.i.g.h.t.

Julianna Lisziewicz

Franco Lori

Jianqing Xu

A molecular delivery complex specific to antigen-presenting cells is formed from a non-viral gene delivery system complexed with foreign genetic material. The complex then enters the targeted cells through a specific receptor and overcomes the degradation mechanism, so that functional uptake of the foreign genetic material, or transduction, of the cell, results in gene expression. The invention also includes a method for genetic immunization without a needle.

Method of delivering genes into antigen presenting cells of the skin

A plasmid DNA that encodes one or more antigenic genes operably linked to a promoter and a truncated retroviral 3' LTR exhibits both enhanced safety and acceptable efficiency of expression of antigenic proteins.

DNA composition and uses thereof

A molecular delivery complex specific to antigen-presenting cells is formed from a a non-viral gene delivery system complexed with foreign genetic material. The complex then enters the targeted cells through a specific receptor and overcomes the degradation mechanism, so that functional uptake of the foreign genetic material, or transduction, of the cell, results in gene expression. The invention also includes a method for genetic immunization without a needle.

Composition for delivering DNA into antigen presenting cells

A method for treating HIV infection in human beings comprising the step of measuring viremia, and if viremia is less than 50,000-100,00 copies per milliliter, administering a combination of compounds selected from the group consisting of hydroxyurea, and one or more reverse transcriptase inhibitors.

Method of inhibiting human immunodeficiency virus using hydroxurea and a reverse transcriptase inhibitor in vivo

The present invention relates to viral inhibition, particularly HIV inhibition, by DNA sequences including multiple target response elements. The DNA construct of the present invention comprises a vector and a promoter operably linked to at least one target response element and, optionally, to other viral inhibitory elements, so that the elements are transcribed in tandem. The DNA construct can be used in the treatment of viral infections, HIV in particular, by obtaining cells from an HIV-infected patient, transforming the cells with the construct and administering the transformed cells to the patient. The protective gene product will only be expressed if the cell becomes infected and a viral protein is made.

Vector with multiple target response elements affecting gene expression

The subject invention provides methods for improving the efficiency of gene transfer to a target cell. The methods are particularly advantageous in that they facilitate the transduction of quiescent cells. In a preferred embodiment the efficiency of gene transfer using a viral vector is enhanced by transiently loading the dytosol of the target cell with dNTP and by transiently adding viral receptors to the surface of the target cell.

Materials and methods for gene transfer

A method for inhibiting replication of reverse transcriptase dependent virus in plant or animal cells, comprising the step of administering to said cells a combination of compounds selected from the group consisting of hydroxyurea, a nucleoside analog, and a protease inhibitor.

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Bethesda, MD, United States of America

Julianna Lisziewicz