Duke University

Regado Biosciences, Inc.

Ohio State Innovation Foundation

Juliana Layzer

Christopher P Rusconi

Bruce A Sullenger

Douglas Brooks

Steven Zelenkofske

Shahid M Nimjee

George A Pitoc

Sabah Oney

Nanette L S Que-Gewirth

Provided herein are aptamers capable of inhibiting the activity of Von Willebrand Factor (VWF). Pharmaceutical compositions comprising these aptamers are also provided. Methods of preventing blood clot formation in a subject by administering the aptamers are provided and methods of treating a blood clot by administering a VWF-targeting agent are also provided.

Von Willebrand Factor (VWF)-targeting agents and methods of using the same

Von Willebrand Factor (VWF)&#x2014;targeting agents and methods of using the same

The present invention relates, in general, to a pharmacologic system to modulate the biology of platelets based upon a nucleic acid ligand that can interact with and modulate the activity of platelet glycoprotein GPVI to regulate platelet function. These nucleic acid ligands are also actively reversible using a modulator that inhibits the activity of the nucleic acid ligand to neutralize this pharmacologic effect and thereby restore GPVI function, including collagen binding, platelet adhesion, collagen-induced platelet activation, and collagen-induced platelet aggregation. The invention further relates to compositions comprising the nucleic acid ligand, the ligand and a modulator, methods to generate the nucleic acid ligand and its modulator, as well as methods of using these agents and compositions in medical therapeutic and diagnostic procedures.

Nucleic acid modulators of glycoprotein VI

Focused aptamer libraries are constructed in accordance with a proteome (i.e., complex mixture of native biomolecules). The libraries may be screened to identify one or more candidate aptamers with desired biological activities other than specific binding to a target. Aptamers which are selected or derivatives thereof may be used for those specific activities in biological systems. Any combination of deconvoluting a focused library (functional profiling), increasing frequencies of particular aptamers in a focused library (Laser SELEX), and decreasing frequencies of particular aptamers in a focused library (DeSELEX) may be performed prior to assaying biological activity.

Focused libraries, functional profiling, laser SELEX, and DESELEX

Growing community of inventors

Durham, NC, United States of America

Juliana Layzer