US Government As Represented by the Secretary of the Army

Julian H Reed

Donald M Cropek

In one embodiment, a hydrogel-enzyme construct for performing high temperature enzymatic reaction on paraoxon, and/or for performing enzymatic reaction on paraoxon following exposure to high temperature, includes a hydrogel having multiple layers of poly(methacrylic acid) (PMAA) and a plurality of dPTE2 enzyme molecules. Individual dPTE2 enzyme molecules are embedded between adjacent PMAA layers and are covalently bonded with respective individual PMAA layers. The hydrogel-enzyme construct is capable of performing enzymatic reaction on the paraoxon when the paraoxon is exposed to the hydrogel-enzyme construct under a temperature condition of up to above 99° C. and below 100° C. or when the paraoxon is exposed to the hydrogel-enzyme construct after the hydrogel-enzyme construct has been heated to a temperature condition of up to 550° C., where the enzymatic reaction on the paraoxon by individual dPTE2 molecules embedded within the hydrogel occurs at a residual activity of between 20% and 100%.

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Champaign, IL, United States of America

Julian H Reed