The United States of America As Represented by the Administrator of the

University of Pittsburgh

Julian G Schiller

James C Fletcher

Chung Chiun Liu

Chung-Chiun Liu

Lemuel B Wingard, Jr

A method for potentiometric detection of hydrogen peroxide including providing an electrolytic cell having a working electrode and a reference electrode. The working electrode is composed of a hydrogen peroxide sensitive material and also is employed as a support for an oxidase enzyme. Introducing a hydrogen peroxide releasing substance into the cell and releasing the hydrogen peroxide by effecting interaction with the enzyme. Developing in said cell an electrical potential which is a function of the concentration of the hydrogen peroxide and measuring the electrical potential of the cell. The method may be employed as a quantitative glucose detector by employing as the enzyme or enzymes glucose oxidase with or without catalase. Apparatus for potentiometric determination of hydrogen peroxide includes a cell containing an electrolyte, a working electrode and a reference electrode. An electrometer is operatively associated with the electrodes. The working electrode is composed of a material which will create an electrical potential when it interacts with hydrogen peroxide. At least one oxidase enzyme is immobilized on the working electrode.

Potentiometric detection of hydrogen peroxide and apparatus therefor

Phenol or a phenol related compound is detected in an electrochemical cell comprising a cathodic compartment containing a cathode in an electrolyte solution containing ferrocyanide ion and an anodic compartment containing an anode formed by coating a gel containing a phenol or phenol related compound oxidizing enzyme onto the conductive substrate of said anode immersed into an electrolyte solution containing ferrocyanide ion into which pure oxygen is passed; means for maintaining a conductive relationship between anodic and cathodic compartments; and means connected to said anode and cathode for measuring the potential of the oxidation of said phenol or related compound.

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Pittsburgh, PA, United States of America

Julian G Schiller