North Carolina State University

American National Red Cross

Pathogen Removal and Diagnostic Technologies Inc.

Pathogen Removal and Diagnostics Technologies Inc.

Julia Tait Lathrop

David John Hammond

Ruben Guillermo Carbonell

Larisa Cervenakova

Iwona Fijalkowska

Jolly Sarkar

Liliana Gheorghiu

David J Hammond

David Hammond

Ligands that bind to prion proteins and methods for using the ligands for detecting or removing a prion protein from a sample, such as a biological fluid or an environmental sample. The ligands are capable of binding to one or more forms of prion protein including cellular prion protein (PrPc), infectious prion protein (PrPsc), and recombinant prion protein (PrPr). Prions from various species, including humans and hamsters, are bound by the ligands. Also provided is a method of treating or retarding the development of a prion-associated pathology in a subject.

Prion protein ligands and methods of use

The invention provides an isolated or purified peptide that binds at least one plasma protein. In one embodiment, the isolated or purified peptide binds to fibrinogen, comprises no more than 10 amino acids, and comprises an amino acid sequence Xaa-Xaa-Xaa-Xaa-Xaa, an amino acid sequence Gly-Xaa-Arg-Xaa, or an amino acid sequence selected from specific amino acid sequences provided herein. Alternatively, the isolated or purified protein binds to &#x3b1;l proteinase inhibitor and/or a protein complex comprising Apo-A1 lipoprotein and paraoxonase. The peptide comprises no more than 10 amino acids and comprises an amino acid sequence Xaa-Xaa-Xaa-His-Xaa-Xaa, and amino acid sequence His-Xaa-Xaa-Xaa-Xaa-Xaa, or an amino acid sequence selected from specific amino acid sequences provided herein. In addition, the invention provides isolated or purified peptide that binds to von Willebrand Factor. The peptide comprises an amino acid sequence Xaa-Xaa-Xaa, an amino acid sequence Tyr-Leu-Xaa-Xaa-Xaa-Thr, or an amino acid sequence selected from specific amino acid sequences provided herein.

Plasma protein-binding ligands

The invention provides a method of characterizing a target that binds to a ligand. The method comprises providing ligands, optionally attached to a support, and contacting the ligands with targets to allow at least one target to bind to at least one ligand. The method further comprises immobilizing the resulting complexes in a first matrix, such that each complex has a different position within the first matrix, and transferring the target of the complex to a second matrix. The position of the target within the second matrix corresponds to the position of the ligand-support complex within the first matrix. The target on the second matrix is then detected.

Method for detecting ligands and targets in a mixture

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Falls Church, VA, United States of America

Julia Tait Lathrop