Leland Stanford Junior University

Jukka Pajarinen

Stuart Barry Goodman

Tzuhua Dennis Lin

Provided are compositions and methods for production of anti-inflammatory cytokines, growth factors, or chemokines. Provided are nucleic acids (e.g., expression vectors) that include an NF&#x3ba;B inflammation response element operably linked to a nucleotide sequence encoding an anti-inflammatory cytokine (e.g., IL-4). In some cases, the nucleic acid is an expression vector selected from: a linear expression vector, a circular expression vector, a plasmid, and a viral expression vector. Also provided are cells (e.g., mesenchymal stem cells&#x2014;MSCs) comprising a nucleic acid that includes an NF&#x3ba;B inflammation response element operably linked to a nucleotide sequence encoding an anti-inflammatory cytokine. In some cases, the nucleic acid is integrated into the cell's genome. Also provided are methods for treating an individual having an inflammation-associated ailment, which can include administering an MSC to the individual, where the MSC includes an NF&#x3ba;B inflammation response element operably linked to a nucleotide sequence encoding an anti-inflammatory cytokine.

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Stanford, CA, United States of America

Jukka Pajarinen