Tosoh Corporation

Juichi Saitoh

Takahiko Ishiguro

Tetsuya Ishizuka

Ryuichi Horie

Toshinori Hayashi

Takashi Yamamoto

Takumi Tokunaga

Akihiro Yokoyama

Masami Otsuka

Yoshifumi Kurihara

Teruhiko Inoue

Yukio Sugiura

Toshitaka Taya

A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once and

Method of assay of target nucleic acid

An oligonucleotide useful for detection of an RNA derived from HIV-1 consisting of at least 10 consecutive bases in any of SEQ ID NOS:1, 2, 4 to 10 and 13 to 17, which can bind to a specific site of the RNA.

Oligonucleotide for detection of HIV-1 and detection method

A method for detecting fluorescence from a liquid sample housed in a transparent or translucent sample container includes providing a sample container in a sample holder, which is opaque except for a sample container introduction opening and incoming and outgoing openings for excitation light; layering a liquid sample and a shielding liquid unmixable therewith to prevent external light from entering through the sample container introduction opening; introducing excitation light from such a direction that the excitation light can irradiate the liquid sample before irradiating the shielding liquid; and detecting fluorescence, which emits in a direction of avoiding absorption by the shielding liquid.

Fluorescence detection method capable of making measurement under external light

Nucleic acid probe

An oligonucleotide for detection or amplification of a gene selected from the group consisting of thermostable direct hemolysin-related hemolysin genes (trh1 and trh2) and thermostable direct hemolysin gene (tdh2) or RNA derived therefrom is provided. Further, method for detecting trh1, trh2 or tdh2 using said oligonucleotide is provided.

Oligonucleotides for detection of Vibrio parahaemolyticus and detection method for Vibrio parahaemolyticus using the same oligonucleotides

A method for assaying a target nucleic acid, comprising: providing an RNA amplification system comprising producing a double-stranded DNA using, as a template, a target RNA containing a specific nucleotide sequence in a sample, said double-stranded DNA having a promoter sequence and being capable of transcribing an RNA comprising the specific nucleotide sequence or a sequence complementary to the specific nucleotide sequence, producing an RNA transcription product comprising the specific nucleotide sequence or a sequence complementary to the specific nucleotide sequence in the presence of an RNA polymerase, and producing the double-stranded DNA using the RNA transcription product as a template, in the presence of a probe labeled with an intercalating fluorochrome having a sequence complementary to the RNA transcription product; measuring the fluorescence intensity in the RNA amplification system with time; calculating a time when the fluorescence intensity satisfies a prescribed criterion based on the measured change in the fluorescence intensity with time; and determining a concentration of the target nucleic acid in the sample based on the calculated time.

Method for assaying nucleic acid

A method of specifically cleaving a double-stranded DNA (a target nucleic acid) at a specific nucleic acid sequence, which comprises irradiating a solution containing at least the target nucleic acid, a nucleic acid probe (a single-stranded oligonucleotide) linked to an intercalater and spermine with light with an absorption wavelength of the intercalater.

Method of cleaving specific nucleic acid sequence

A method of assay of a specific nucleic acid anticipated in a sample, which comprises:

Method of assay of nucleic acid sequences

A method of measuring a melting temperature of a nucleic acid, which comprises a step of monitoring the fluorescent intensity of a mixture of a sample and a probe which is labeled with a fluorescent intercalative dye and contains a base sequence complementary to a specific nucleic acid in the sample, while varying the temperature of the mixture.

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Kanagawa, Japan

Juichi Saitoh