Academia Sinica

National Chung Hsing University

Trade Wind Biotech Co., Ltd.

Jui-Jen Chang

Chieh-Chen Huang

Wen-Hsiung Li

Cheng-Yu Ho

Ming-Che Shih

Yu-Ju Lin

Hao-Yeh Lin

Caroline Thia

Yi-Chen Wang

Feng-Ju Ho

Hsing-Yu Huang

Wei-En Chen

Jiunn-Jyi Lay

Hui-Min Wang

Chia-Hung Chou

Chang-Lung Han

Anandharaj Marimuthu

Rizwana Parveen Rani Mohamed Gobil

The invention discloses an astaxanthin (AST) nanoemulsion and its manufacturing method. The manufacturing method comprising steps of: adding an AST material into a peanut oil and mixing them uniformly to obtain an AST oil; adding 0.25-1.5 (w/w) % of a surfactant into the AST oil and mixing them uniformly to obtain a mixed solution; and adding water into the mixed solution to obtain an AST emulsion precursor; and shaking the AST emulsion precursor to obtain the AST nanoemulsion.

Astaxanthin nanoemulsion and manufacturing method thereof

The present disclosure relates to a recombinant protein comprising a plurality of type II cohesin repeats. The present disclosure provides a recombinant cellulosome complex comprising: the recombinant protein comprising a plurality of type II cohesin repeats; a recombinant cellulosome complex integrating protein A comprising a plurality of type I cohesin repeats, a plurality of cellulose-binding modules and a type II dockerin; and a plurality of recombinant enzymes each comprising a type I dockerin. A cell, a method for digesting a cellulose and a method for producing ethanol are also provided.

Recombinant cellulosome complex and uses thereof

Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form.

Recombinant polynucleotide sequence for producing astaxanthin and uses thereof

Described herein is an engineeredcell, the cell comprising in its genome: (i) two different nucleic acid molecules that each contain a promoter operably linked to a gene encoding a functional enzyme, and (ii) a selection nucleic acid molecule that contains a promoter operably linked to a gene encoding a selectable marker, wherein all of the nucleic acids molecules of (i) and (ii) are in tandem and the engineered cell expresses all of the proteins encoded by the genes of (i) and (ii).

Engineered yeast for production of enzymes

Described is a method of introducing multiple nucleic acid molecules into the genome of a cell. The method includes providing a plurality of nucleic acid molecules, each of the plurality of nucleic acid molecules containing (a) a nucleic acid sequence operatively linked to a promoter sequence at the 5' end of the nucleic acid molecule, and (b) an overlapping sequence at the 3&#x2032; end of the nucleic acid molecule.

Method for ordering and introducing multiple genes into a genome

Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds.

Flavor compound-producing yeast strains

An expression system for producing a multi-enzyme complex, the system including a nucleic acid molecule containing a promoter operatively linked to a nucleotide sequence including multiple genes encoding multiple enzymes that are components of the multi-enzyme complex.

Expression system for producing multi-enzyme complexes and uses thereof

A polycistronic expression cassette encoding proteins necessary for constructing a multi-enzyme complex was developed. Also disclosed herein is a host cell containing this polycistronic expression cassette and uses thereof in degrading biomass.

Method for producing extracellular multi-enzyme complexes in host cells

The present invention provides a microbial hydrogen-producing process, comprising: providing at least onemicrobe and at least onemicrobe; and co-culturing said at least onemicrobe and said at least onemicrobe in a fermentation culture system to produce hydrogen. The present invention also provides a microbial hydrogen-producing system, characterized by that the system comprises at least onemicrobe and at least onemicrobe, and it uses an organic waste medium as the substrate to perform a hydrogen-producing fermentation having high efficiency, high stability, and high reproducibility.

Growing community of inventors

Kaohsiung, Taiwan

Jui-Jen Chang