Glykos Finland Oy

Vtt Technical Research Centre of Finland Ltd

Suomen Punainen Risti Veripalvelu

Nestec S.a.

Hankkija Oy

Juhani Saarinen

Jari Natunen

Tero Satomaa

Markku Saloheimo

Anne Kanerva

Ann Westerholm-Parvinen

Anne Huuskonen

Christopher Landowski

Anna-Liisa Hänninen

Noora Salovuori

Jari Helin

Annamari Heiskanen

Merja Elisa Penttila

Merja Penttilä

Jukka Hiltunen

Heidi Anderson

Maria Blomqvist

Ulla Impola

Anne Olonen

Milla Mikkola

Jarmo Laine

Taina Jaatinen

Bernhard Helk

Filip S Ekholm

Johanna Nystedt

Jukka Partanen

Hanna Salo

Heli Viskari

Christian Ostermeier

Benjamin Patrick Sommer

Ramon Wahl

Karl-Anders Karlsson

Jussi Joensuu

Anja Vilkman

Heidi Virtanen

Ritva Niemela

Minna Tiittanen

Anne Leppänen

Susann Teneberg

Heidi Salminen

Halina Miller-Podraza

Georg Schmidt

Eero Mustalahti

Janica Wakkinen

Merja Penttila

Ritva Niemelae

Anna-Liisa Hanninen

Ritva NiemelÄ

Jonas Angström

Maan Abul-Milh

Niamh Roche

The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.

Protease deficient filamentous fungal cells and methods of use thereof

Hydrophilic linkers and their conjugates are disclosed. Formula (I) (II)

Hydrophilic linkers and conjugates thereof

A molecule comprising a saccharide bound via an O-glycosidic bond to a hydroxyl group of a toxic payload molecule is disclosed. An antibody-drug conjugate comprising an antibody covalently bound to a toxic payload molecule, optionally via a linker group, and a saccharide bound via an O-glycosidic bond to a hydroxyl group of the toxic payload molecule is further disclosed.

Saccharide derivative of a toxic payload and antibody conjugates thereof

The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such ascells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing &#x3b1;1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

Production of glycoproteins with mammalian-like N-glycans in filamentous fungi

Described herein are compositions including filamentous fungal cells, such asfungal cells, having reduced protease activity and expressing fucosylation pathway. Further described herein are methods for producing a glycoprotein having fucosylated N-glycan, using genetically modified filamentous fungal cells, for example,fungal cells, as the expression system.

Production of fucosylated glycoproteins

The invention relates to a composition comprising a glycoprotein comprising the Fc domain of an antibody, or a fragment thereof, comprising an Asn (asparagine) residue and an oligosaccharide structure attached thereto, wherein said oligosaccharide structure has a structure according to formula I; and wherein at least 20% of the oligosaccharide structures attached to glycoprotein in the composition consist of oligosaccharide structures according to formula (I).

Glycoprotein

The invention describes methods for production of novel composition of glycans, glycomes, from human multipotent stem cells. The invention is further directed to methods for modifying the glycomes and analysis of the glycomes and the modified glycomes. Furthermore the invention is directed to stem cells carrying the modified glycomes on their surfaces.

Carbohydrate profile compositions from human cells and methods for analysis and modification thereof

The present invention is directed to a method of production of an immunostimulatory composition having a saccharide fraction, the method including the steps of hydrolyzes of yeast cells and recovering the soluble fraction. The saccharide fraction having composition thus obtained may be incorporated as a food or beverage component or be used as a pharmaceutical for treatment of specific conditions.

Production of a saccharide composition comprising glucans and mannans by alkaline and acid hydrolysis of yeast cells

The present invention reveals novel methods for producing novel carbohydrate compositions, glycomes, from animal tissues. The tissue substrate materials can be total tissue samples and fractionated tissue parts, or artificial models of tissues such as cultivated cell lines. The invention is further directed to the compositions and compositions produced by the methods according to the invention. The invention further represent methods for analysis of the glycomes, especially mass spectrometric methods.

Tissue carbohydrate compositions and analysis thereof

The invention relates to a method for culturing human embryonic stem cells (hESCs) and/or induced pluripotent stem (iPS) cells on a lectin. The invention relates also to the use of a lectin in a method for culturing human embryonic stem cells (hESCs) and/or induced pluripotent stem (iPS) celts and a culture medium composition containing a lectin attached on the culturing plates.

Culture of cells

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Helsinki, Finland

Juhani Saarinen