Nch Corporation

Judy Pruitt

Charles J Greenwald

Gabriel F K Everett

Jordan E Church

Amanda K Rosmarin

Daniel Aberle

George Aboagye

Lester Levy

Haibo Cao

Christopher Zetena

Kelly A Gillespie

Skylar Rae White

Amanda Rosmarin

A composition and method for activating probiotic spores in food and beverage products, such as steeped teas, coffee, soups, and sauces. A nutrient-germinant composition comprises one or more L-amino acids, optionally one or more buffers to maintain the pH of the composition when added to water in a range of around 6-8, optionally D-glucose, D-fructose, or both D-glucose and D-fructose, and optionally, an osmoprotectant. The nutrient-germinant composition, one or more species ofspores, and a food or beverage product may be pre-mixed in any combination. Water is added to the mixture and heated to a temperature range of 42 to 100&#xb0; C. to germinate the probiotic spores prior to being consumed.

Composition and method for germinative compounds in probiotic food and beverage products for human consumption

A method for improving the quality of pond water used in aquaculture applications by adding to the pond water active bacteria that are preferably germinated from spores on site using a nutrient-germinant composition and an incubation method for increased spore germination efficiency, in combination with a nitrification enhancement agent such as calcium carbonate or calcified seaweed, and an optional reaction surface area modifier such as calcified seaweed or plastic or metal particles or fragments. The nutrient-germinant composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores (preferably of one or morespecies) or they may be separately combined for germination. The incubation method comprises heating a nutrient germinant composition and bacteria spores, to a temperature range of 35&#xb0; C. to 60&#xb0; C. for around 2 to 60 minutes to produce an incubated bacteria solution that is discharged to the aquaculture application.

Method for improving quality of aquaculture pond water using a nutrient germinant composition and spore incubation method

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or morespecies, and heating to a preferred elevated temperature range of 41&#xb0; C. to 44&#xb0; C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

Nutrient rich germinant composition and spore incubation method

Growing community of inventors

Mesquite, TX, United States of America

Judy Pruitt