Anadys Pharmaceuticals, Inc.

Scriptgen Pharmaceuticals, Inc.

Judith M Healy

C Richard Wobbe

John D Bradley

Craig M Thompson

Jeffrey B Moore

Caroline E Donnelly

Jason A Thresher

Kelvin T Lam

Yueh-Tyng Chien

Andrea J Lesoon

Jana Bodorova

Patricia Carroll

The present invention provides an isolated Staphylococcal GTPase obg gene and a Staphylococcal GTP-binding protein encoded thereby. A recombinant expression vector and an engineered host cell are also provided that include a polynucleotide encoding a Staphylococcal GTP-binding protein. The present invention further relates to a method of producing Staphylococcal GTP-binding protein and a method for high throughput screening to identify potential antimicrobial compounds useful against Staphylococcal bacterial strains.

Staphylococcal GTPase obg nucleotide sequence encoding Staphylococcal GTP-binding protein

The present invention relates to a recombinant organism, and a bacterial strain such as Staphylococcus, and , in particular. The organism has a regulatable gene for encoding an RNA polymerase specificity factor required for expression of at least one gene essential for growth of the organism. The regulatable gene is one which is responsive to an exogenous effector molecule, and may in particular be an inducer-responsive gene including an operator site, such as a lac operator, to which a repressor, such as a lacl-encoded repressor, is capable of binding. The regulatable gene may in particular be an IPTG responsive plaC allele.

Staphylococcus strains comprising an inducible gene encoding an RNA polymerase specificity factor

The invention provides novel yeast cells comprising genes whose expression can be modulated by growth in the presence or absence of metal ions, methods for making such yeast cells, and methods of using such yeast cells for determining the requirement for expression of particular genes for the growth or viability of the yeast cells.

Regulated gene expression in yeast

The present invention provides isolated nucleic acids encoding RNA polymerase alpha subunit from vectors comprising the nucleic acids, cells comprising the vectors, and methods for producing alpha subunit. The invention also provides in vitro and in vivo methods for high-throughput screening to identify inhibitors of RNA polymerase.

M. tuberculosis RNA polymerase alpha subunit

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Lexington, MA, United States of America

Judith M Healy