Monsanto Technology LLC

Monsanto Company

Monsanto Technologies LLC

Judith C Donovan

William P Donovan

Annette C Slaney

John W Pitkin

James T Engleman

James Arthur Baum

Thomas M Malvar

James Kevin Roberts

Karina Krasomil-Osterfeld

James Engleman

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space fromand related strains. The proteins are isolated from culture supernatants ofand related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle () and Southern Corn Rootworm (). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

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The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space fromand related strains. The proteins are isolated from culture supernatants ofand related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

Disclosed arestrains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae () and Japanese beetle larvae (). Also disclosed are novelcrystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Disclosed are transgenic plants, seeds, and progeny from such plants comprising novel nucleic acid sequences encoding both CryET33 and CryET34 crystal proteins. The novel nucleic acid sequences comprise SEQ ID NO:11. CryET33 and CryET34 crystal proteins comprise SEQ ID NO:3 and SEQ ID NO:4 and exhibit insecticidal activity against coleopteran insects including red flour beetle larvae () and Japanese beetle larvae ().

Insect-resistant transgenic plants transformed with CryET33 and CryET34-encoding nucleic acids

Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor

Disclosed are strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae ( ) and Japanese beetle larvae ( ). Also disclosed are novel crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Bacillus thuringiensis cryet33 and cryet34 compositions and uses thereof

Disclosed are,strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (,) and Japanese beetle larvae (,). Also disclosed are novel,crystal toxin genes, designated cryET33 and cryET34, which encode respectively the coleopteran-toxic proteins, CryET33 (29-kDa) crystal protein, and CryET34 (14-kDa) crystal protein. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Bacillus thuringiensis CryET33 and CryET34 compositions and uses therefor

Disclosed are,strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (,) and Japanese beetle larvae (,). Also disclosed are novel,crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Bacillus thuringiensis CryET33 and CryET34 compositions and uses thereof

Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Growing community of inventors

Levittown, PA, United States of America

Judith C Donovan