Thermo Fisher Scientific Baltics Uab

Fermentas Uab

Judita Lubiene

Arvydas Lubys

Arvydas Janulaitis

Arturas Berezniakovas

Jolanta Vitkuté

Enzyme compositions and their method of use that provide ready-to-use master mixtures. The compositions comprise a modified thermophilic DNA polymerase lacking 5'-3&#x2032; and 3&#x2032;-5&#x2032; exonuclease activity premixed with T4 DNA polymerase, Klenow fragment and T4 polynucleotide kinase and all other necessary components, including reaction buffer and nucleoside triphosphates, required to perform DNA blunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps. Among other benefits, the mixture of different enzymes, buffers and nucleoside triphosphates is stable during prolonged storage.

Enzyme composition for DNA end repair, adenylation, phosphorylation

Provided is a methylation-specific restriction endonuclease for a DNA duplex substrate, which endonuclease recognizes in a strand of the duplex a 2 to 6 nucleotide recognition sequence comprising a 5-methylcytosine, and cleaves each strand of the duplex at a fixed position outside the recognition sequence.

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Vilnius, Lithuania

Judita Lubiene