Cyanamid Iberica, S.a.

Juan Plana Duran

Jose Ignacio Casal Alvarez

Luis Enjuanes Sanchez

Maria Muntion Saenz

Joaquin Castilla Castrillon

José Manuel Sanchez Morgado

Ana Mendez Zunzunegui

MaLuisa Ballesteros Jarreno

Zoltán Penzes

José Manuel Gonzalez Martinez

Carlos Miguel Sanchez Sanchez

Cristina Smerdou Picazo

Isabel Sola Gurpegui

Sara Alonso Villanueva

Ander Izeta Parmesan

Isabel Climent Sanchez

The vectors comprise a recombinant defective viral genome expressing at least one antigen suitable for the induction of systemic and secretory immune responses or an antibody conferring protection against an infectious agent. The defective viral genome comprises the genome of a parental virus having the viral replicase recognition signals located on ends 3' and 5&#x2032;, further comprising internal deletions, and wherein said defective viral genome depends on a helper virus for its replication and encapsidation. These vectors are suitable for the forming of a recombinant system comprising the aforesaid expression vector, and a helper virus. The system is suitable for the manufacture of mono- and polyvalent vaccines against infectious agents of different animal species, especially pigs, dogs and cats, and as expression vehicles for antibodies protective against infectious agents.

Vectors based on recombinant defective viral genomes, and their use in the formulation of vaccines

Recombinant proteins of the causative virus of porcine reproductive and respiratory syndrome (PRRS), corresponding to ORFs 2 to 7 of the PRRSV Spanish isolate (PRRS-Olot), have been produced in baculovirus expression system using Sf9 cell cultures as a permissive host. These recombinant proteins are suitable for the formulation of vaccines capable of efficaciously protecting porcine livestock from PRRS and for the preparation of diagnostic kits adequate for detection of anti-PRRSV antibodies as well as of PRRSV in a pig biological sample. This invention is of interest to Veterinary Medicine.

Recombinant PRRSV proteins, diagnostic kits and vaccines containing such

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Vall de Bianya-Olot, Spain

Juan Plana Duran