Glaxosmithkline Biologicals Sa

Juan Jose Diaz Garcia

Kent Raymond Myers

Chad Austin Farrenburg

Ahmad Taimour Baig

Jeri Kay Sandvick

Jeb Yeatts Vandenburg

Lora Lea Lawrence

Felicie Georgette Colette Denet

Margit Holzer

Margit Theresia Holzer

Saponin extracts containing at least 93% QS-21 main peak and 0.25-3% 2018 component by UV absorbance at 214 nm, methods for making said extracts, their use as vaccine adjuvants and related aspects.

Saponin purification

Crude aqueous extracts ofMolina containing at least the QS-21 main peak and 2018 component, wherein the ratio of 2018 component/QS-21 main peak is ≤0.075, as measured by UV absorbance at 214 nm, methods for obtaining such extracts and related aspects.

Saponin extraction

Crude aqueous extracts ofMolina containing at least the QS-21 main peak and 2018 component, wherein the ratio of 2018 component/QS-21 main peak is &#x2264;0.075, as measured by UV absorbance at 214 nm, methods for obtaining such extracts and related aspects.

The present disclosure concerns methods for producing purified saponins, such as QS-21, which reduces the number of necessary lyophilization cycles to one by a step of solvent exchange and optional subsequent exposure of the purified saponin product to at least one exposure to dry gas followed by exposure to a vacuum to further rid the purified saponin product of residual solvent molecules.

Growing community of inventors

Hamilton, MT, United States of America

Juan Jose Diaz Garcia