Perkinelmer Health Sciences, Inc.

Biomotif Ab

Hans Jornvall

Juan Astorga-Wells

Harold Philip Swerdlow

Sang-Ryoul Park

Ulf Lindberg

Tomas Bergman

Mats Jonsson

The present invention relates a use of the electrocapture-based separation technology combined with mass spectrometrical fragmentation methods, e.g. sequence of polypeptides by collision-induce dissociation mass spectrometry, for the identification and/or characterization molecules of interest. It also relates the combination of the electrocapture-base separation technology with other liquid separation methods, as e.g. liquid chromatography, in order to achieve multi-dimensional separations prior mass spectrometrical analysis. In addition, it relates physical interfaces between electrocapture-based separations and different types mass spectrometers for on-line or off-line analysis, as well as the coupling of electrocapture-based separations, liquid chromatography and different types of mass spectrometrometers.

Methods and interfaces for single and multidimensional separations for characterization and/or identification of molecules by mass spectrometry

The present invention relates a use of the electrocapture-based separation technology combined with mass spectrometry (e.g. sequence of polypeptides by collision-induce dissociation mass spectrometry, for the identification and/or characterization molecules of interest). In addition, it relates physical interfaces between electrocapture-based separations and different types mass spectrometers for on-line analysis, as well as the coupling of electrocapture-based separations, liquid chromatography and different types of mass spectrometrometers. It also relates the combination of the electrocapture-base separation technology with other liquid separation methods, as e.g. liquid chromatography, in order to achieve multidimensional separations prior mass spectrometrical analysis. The invention also relates to a separation device comprising a capture device, a fluidic connector e.g. an electrospray source, an electrospray interface-source and a mass spectrometer.

The invention relates to a method for capturing charged molecules of interest traveling in an electrolyte flow stream through an electrically non-conductive channel, comprising at least one anode and at least one cathode individually separated from said channel, but in electrical contact with said flow stream, by a conductive ion selective semi-permeable membrane. Said membrane interferes with the normal migration of ions towards its respective electrode, generating at least two zones of different electric field. Balance between hydrodynamic and electrical forces captures certain ions into the flow stream. it also relates to a device for performing the method.

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Skarpnack, Sweden

Juan Astorga-Wells