Zeneca Limited

Imperial Chemical Industries Limited

Katholieke Universiteit Leuven, Ku Leuven R&d

K.u. Leuven Research & Development

Jozef Vanderleyden

Willem F Broekaert

Sarah Bronwen Rees

Bruno Philippe Cammue

Rupert W Osborn

Franky Rg Terras

Miguel De Bolle

Bruno Pa Cammue

Dirk De Vos

Viola Rosemeyer

Erik Van Der Eycken

Hans Steenackers

Bruno P A Cammue

Bruno Philippe Angelo Cammue

Franky R G Terras

Bharat S Savaliya

Denis Ermolatev

Sigrid De Keersmaecker

Bruno Phillippe Cammue

Bharat Savdasbhai Savaliya

The invention relates to substituted 2-aminoimidazoles and their imidazo[1,2-a]pyrimidinium salts precursors being active against biofilm formation. The invention also relates to imidazo[1,2-a]pyrimidinium salts bearing an azidoalkyl substituent, and to substituted 2-aminoimidazoles wherein the amino group bears a terminal heterocyclic group such as a triazolyl group which are formed through azide-alkyne Huisgen cycloaddition starting from said imidazo[1,2-a]pyrimidinium salts bearing an azidoalkyl substituent. The invention also relates to a class of N-(azidoalkyl)pyrimidin-2-amines useful as starting materials for the synthesis of said imidazo[1,2-a]pyrimidinium salts bearing an azidoalkyl substituent. The invention also relates to antimicrobial compositions that include a microbial biofilm formation inhibiting amount of such substituted 2-aminoimidazoles or imidazo[1,2-a]pyrimidinium salts in combination with excipients. Methods for inhibiting or controlling microbial biofilm formation in a plant, a body part of a human or an animal, or a surface with which a human or an animal may come into contact are also disclosed.

Compounds, compositions, and methods for controlling biofilms

Biocidal proteins capable of isolation from seeds have been characterized. The proteins have an amino acid sequence containing the common cysteine/glycine domain of Chitin-binding Plant Proteins but show substantially better activity against pathogenic fungi, a higher ratio of basic amino acids to acidic amino acids, and/or antifungal activity which results in increased hyphal branching. Antimicrobial proteins isolated from Amaranthus, Capsicum, Briza and related species are provided. The proteins show a wide range of antifungal activity and are active against Gram-positive bacteria. DNA encoding the proteins may be isolated and incorporated into vectors. Plants may be transformed with this DNA. The proteins find agricultural or pharmaceutical application as antifungal or antibacterial agents. Transgenic plants expressing the protein will show increased disease resistance.

Biocidal proteins

The present invention relates to a new mutant CNPAF 512 strain, having an inactivated raiI gene. Inactivation of the raiI gene leads to increased nodule number in beans upon inoculation thereof with the mutant strain. Based on this specific strain other strains can be mutated in the similar gene(s) in order to enable nodule increase and nitrogen fixation in other Leguminosae species.

Increase of nodule number and nitrogen fixation in leguminosae

Biocidal proteins isolated from seeds have been characterised, in particular proteins isolated from members of the Brassicaceae, Compositae and Leguminosae families including Raphanus, Brassica, Sinapis, Arabidopsis, Dahlia, Cnicus, Lathyrus and Clitoria. The proteins show a wide range of antifungal activity and some are active against Gram-positive bacteria. All share a common amino acid sequence. DNA encoding the proteins has been isolated and incorporated into vectors. Plants transformed with this DNA may be produced. The proteins find commercial application as antifungal or antibacterial agents; transformed plants will show increased disease-resistance.

Transgenic plants expressing biocidal proteins

Biocidal proteins isolated from Mirabilis have been characterized. The proteins show a wide range of antifungal activity and are active against gram-positive bacteria. DNA encoding the proteins has been isolated and incorporated into vectors. Plants transformed with this DNA have been produced. The proteins find commercial application as antifungal or antibacterial agents; transformed plants will show increased disease-resistance.

DNA encoding biocidal proteins

Biocidal proteins isolated from Mirabilis have been characterised. The proteins show a wide range of antifungal activity and are active against gram-positive bacteria. DNA encoding the proteins has been isolated and incorporated into vectors. Plants transformed with this DNA have been produced. The proteins find commercial application as antifungal or antibacterial agents; transformed plants will show increased disease-resistance.

Biocidal proteins from plants

Growing community of inventors

Heverlee, Belgium

Jozef Vanderleyden