Pioneer Hi-bred International, Inc.

Other

Curagen Corporation

Joyce R Maddox

Jacob T Gilliam

Jonathan P Duvick

Otto Folkerts

Oswald R Crasta

Jon Duvick

Xun Wang

Tracy A Rood

Benjamin A Bowen

Aragula Gururaj Rao

Nancy Keller

Edmund H Crane, Iii

Petr Karlovsky

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated fromand. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use

The present invention provides transformed plants comprising a polynucleotide enconding amino polyol amine oxidase (APAO ) fromand a method of expressing APAO transgenic plants and seeds. In this way, transgenic plant can be produced having fumonisin degrading capability, as well as with the capability of producing the degrading enzymes.

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from . Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems.

Amino polyol oxidase amine polynucleotides and related polypeptides and methods of use

Compositions and methods for the complete detoxification of fumonisin and fumonisin degradation products are provided. Particularly, nucleotide sequences corresponding to the detoxification enzymes are provided. The sequences find use in preparing expression cassettes for the transformation of a broad variety of host cells and organisms.

Compositions and methods for fumonisin detoxification

The present invention provides nucleotide sequences encoding zearalenone detoxification polypeptides, and methods for mycotoxin detoxification using the sequences. One method comprises stably incorporating into the genome of a plant cell, a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence.

Compositions and methods of zearalenone detoxification

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from and . Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium. Plants can be regenerated from the transformed plant cells.

Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

Fumonisin detoxification compositions and methods

The invention provides isolated maize lipoxygenase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering lipoxygenase concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

Growing community of inventors

Omaha, NE, United States of America

Joyce R Maddox