Medical Diagnostic Laboratories, LLC

Other

Joyce Eskdale

Grant E Gallagher

Rachael Siegel

Rachel Siegel

Raymond Yu

Jonathan Brazaitis

Nicholas Megjugorac

Jihong Dai

Shekar Srinivas

Adam Swider

The present invention provides a method of specifically detecting IFN-&#x3bb;2 mRNA or IFN-&#x3bb;3 mRNA. There is provided a qRT-PCR method specifically detecting, discriminating and quantifying IFN-&#x3bb;2 and IFN-&#x3bb;3 mRNA in a biological sample obtained from a human. There is provided qRT-PCR methods and primers and probes that specifically detect IFN-&#x3bb;2 mRNA but not IFN-&#x3bb;3 mRNA and vice versa in humans in order to detect, quantify and discriminate IFN-&#x3bb;2 mRNA and IFN-&#x3bb;3 mRNA.

Methods and reagents that specifically detect, distinguish and quantify IFN-LAMBDA2 mRNA from IFN-LAMBDA3 mRNA in humans

The present invention is directed to the identification of a novel repressor located between &#x2dc;1.2 kb to &#x2dc;1.6 kb from the translation start site of the IFN-&#x3bb;1 promoter. The present invention provides a method of using siRNAs against ZEB1 (binds to the repressor region) and BLIMP-1 (binds outside the repressor region) and increases the promoter activity of IFN-&#x3bb;1 (i.e., increases the production of IFN-&#x3bb;1 protein). siRNAs against ZEB1 mRNA or BLIMP-1 mRNA increase IFN-&#x3bb;1 gene activity. There is provided a therapeutic application of siRNAs against ZEB1 and BLIMP-1 mRNAs in treating a mammal (including a human) by increasing the production of IFN-&#x3bb;1 protein that promotes an anti-viral response as well as treats asthma diseases and colon diseases.

Repressor on IFN-&#x3bb; promoter and siRNA against GATA1, EVI1, and CRX to alter IFN-&#x3bb; gene activity

A naturally-occurring soluble truncated IL-23R&#x3b1; protein (i.e., &#x394;9 IL-23R&#x3b1;) is shown to be present in a biological sample and can serve as a diagnostic tool for autoimmune diseases. There is provided an enzyme-linked immunosorbent assay (ELISA) and test kit for the serological detection of the soluble truncated form of IL-23R&#x3b1; protein. More particularly, antibody-sandwich ELISA method and kits for &#x394;9 IL-23R&#x3b1; as an antigen were developed to detect &#x394;9 IL-23R&#x3b1; levels in biological samples from a mammal and a human patient and are used as a diagnostic index. The present disclosed ELISA has utility as a diagnostic tool to detect Crohn's disease in patients using EDTA-plasma.

Elisa for a naturally-occurring soluble truncated form of IL-23 receptor

Methods and reagents that specifically detect, distinguish and quantify IFN-&#x3bb;2 mRNA from IFN-&#x3bb;3 mRNA in humans

The present invention is directed to the identification of a novel repressor located between &#x2dc;1.2 kb to &#x2dc;1.6 kb from the translation start site of the IFN-&#x3bb;1 promoter. The present invention provides a method of using siRNAs against ZEB1 (binds to the repressor region) and BLIMP-1 (binds outside the repressor region) and increases the promoter activity of IFN-&#x3bb;1 (i.e., increases the production of IFN-&#x3bb;1 protein). siRNAs against ZEB1 mRNA or BLIMP-1 mRNA increase IFN-&#x3bb;1 gene activity. There is provided a therapeutic application of siRNAs against ZEB1 and BLIMP-1 mRNAs in treating a mammal (including a human) by increasing the production of IFN-&#x3bb;1 protein that promotes an anti-viral response as well as treats asthma diseases.

Repressor on IFN-&#x3bb; promoter and siRNA against ZEB1 and BLIMP-1 to increase IFN-&#x3bb; gene activity

The present invention provides a method of treating Th2-associated diseases and disorders by modulating the expression or secretion of IL-4, IL-5 and IL-13 using interferon lambda (IFN-&#x3bb;). For Th2-associated diseases and disorders, cells of a patient having a Th2-associated disease or disorder are treated ex vivo, with IFN-&#x3bb; and returned to the patient. The present invention also provides a method of ex vivo treatment, in conjunction with co-administration of IFN-&#x3bb;.

Growing community of inventors

Milltown, NJ, United States of America

Joyce Eskdale