Monsanto Technology LLC

Monsanto Company

Joyce E Fry

Yinghui Dan

Ming Cheng

Hua-ping Zhou

Arlene R Howe

Maud Ann Hinchee

Helen Allison

Xiaorong Feng

Tianci Hu

Fengming Lu

Maxim Radionenko

Yeuchun Wan

Renee Jean Rozman

Mary Jacqueline Fedele

Dannette Vaudrilyn Connor-Ward

Thomas Elmo Clemente

Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.

Method for the production of transgenic plants

Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.

The present invention relates to an improved transformation and regeneration system for wheat. In particular, the invention relates to enhancements in the glyphosate selection system. The transformation method is efficient and reliable for production of fertile plants with improved agronomic qualities.

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The present invention relates to a rapid transformation and regeneration system for plants. In particular, the invention relates to a plant tissue preparation system. The transformation method is efficient and reliable for production of fertile plants with improved agronomic qualities.

Efficiency agrobacterium-mediated plant transformation method

Disclosed are processes for producing stably transformed fertile wheat a system of transforming wheat via. This invention provides methods transforming a variety of explants, such as freshly isolated or pre-cultured immature embryos, embryogenic callus and suspension cells. Also disclosed are methods for recovering transgenic plants after transformation within a short period of time, if the explants are regenerable at the time of transformation. Thus the frequency of somaclonal variation associated with prolonged in vitro culture period is significantly reduced. The transformation frequency using this system is comparable to or better than published methods using other systems, such as microprojectile bombardment.

Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom

A rapid transformation regeneration system is disclosed. This system takes two-three months to obtain transgenic plants. Transformation efficiencies are very high. This system also has been demonstrated with several different selecting systems and is particularly useful for transforming wheat.

Rapid and efficient regeneration of transgenic wheat plants

Methods and materials for the production of transgenic soybeans are disclosed. Preparation of explants from specific regions of soybean seedlings resulted in improved transformation efficiencies. Incubation of soybean seedlings between about 0.degree. C. and about 10.degree. C. prior to preparing explants was found to be further beneficial to the preparation of transgenic soybeans.

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St. Louis, MO, United States of America

Joyce E Fry