USA As Represented by Secretary of the Navy

Joyce A Breger

Igor L Medintz

Kimihiro Susumu

James B Delehanty

Philip E Dawson

Michael H Stewart

Scott Walper

Sebastian A Diaz

Jesper Brask

Markus Muttenthaler

A nanoplatelet serves as a substrate for immobilizing enzymes involved in consecutive reactions as a cascade. This results in a significant increase in the rate of catalysis as well as final product yield compared to non-immobilized enzymes or enzymes immobilized to quantum dots.

Self-assembled nanoplatelet-enzyme bioconjugates providing for increased biocatalytic efficiency

Lipase activity can be detected with a biosensor that includes a quantum dot adhered to a construct having a lipase-cleavable ester to attach a fluorophore acceptor configured as a F&#xf6;rster resonance energy transfer (FRET) acceptor to the QD when the construct is bound thereto. Cleavage of the ester by a lipase results in a measurable reduction in FRET. In further embodiments, the cleavable ester can be used to detect esterase activity, or the ester could be replaced with a glycosidic linkage to detect glycoside activity.

Nanoparticle-based lipase biosensor utilizing a custom-synthesized peptidyl-ester substrate

Nanoparticles (and optionally a cargo such as a drug) can be delivered to cells by attaching just a single dendritic peptide to the nanoparticle. The dendritic peptide includes a polyhisitidine motif and a hinge and a spacer connecting the polyhistidine to a lysine-based dendritic wedge displaying at least two copies of a cell-penetrating peptide motif.

Use of single dendritic wedge cell penetrating peptides to facilitate cellular delivery of nanoparticles and nanoparticles carrying cargos

Growing community of inventors

Washington, DC, United States of America

Joyce A Breger