Nec Solution Innovators, Ltd.

Gunma University

Kanagawa Prefectural Hospital Organization

Nec Soft, Ltd.

Ohtsu, Takashi

Tsuji, Shotaro

Tsuji, Makiko

Jou Akitomi

Iwao Waga

Katsunori Horii

Naoto Kaneko

Shintarou Katou

Hirotaka Minagawa

Masayasu Kuwahara

Ikuo Shiratori

Makio Furuichi

Takashi Ohtsu

Shotaro Tsuji

Hiromi Takenaka

Yoshihito Yoshida

Hirotaka Yagi

Kensaku Nishikata

Ikuo Kajiyama

Tomoko Fujita

Akihisa Shimizu

Mineko Yamaguchi

Shintaro Kato

Tomoko Shiratori

Naomi Hirabayashi

Miho Ishii

Tomoko Murata

Tsuji Makiko

Tsuji Shotaro

Ohtsu Takashi

The present invention provides a novel nucleoside derivative or a salt thereof, a polynucleotide synthesis reagent, a method for producing a polynucleotide, a polynucleotide, and a method for producing a binding nucleic acid molecule. The nucleoside derivative or a salt thereof of the present invention is represented by the following chemical formula (1).  In the chemical formula (1), Su is an atomic group having a sugar skeleton at a nucleoside residue or an atomic group having a sugar phosphate skeleton at a nucleotide residue, and may or may not have a protecting group, Land Lare each independently a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms, Xis an imino group (&#x2014;NR&#x2014;), an ether group (&#x2014;O&#x2014;), or a thioether group (&#x2014;S&#x2014;), and Ris a hydrogen atom or a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms.

Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule

The continual engagement support deviceincludes: the performance data storage meansthat stores performance data including time data indicating the time of performance and sensor data of the time of performance or the surrounding time obtained from the sensors mounted on the portable terminal when the target action is performed via the portable terminal possessed by the target user; the timing detection meansthat detects the performance timing, which is the timing at which the target user is likely to perform the target action during the predetermined cycle, on the basis of the performance data; and the notification meansprovides a notification to prompt the performance of the target action at the detected performance timing when the target action is not performed for a predetermined period of time or longer.

Continual engagement support device, portable terminal, continual engagement support method, and program

The present invention provides a novel molecule that can be used for detection of sIgA. The sIgA-binding nucleic acid molecule of the present invention is characterized in that it binds to secretory immunoglobulin A (sIgA) with a dissociation constant of 37.7 nM or less, and preferably includes a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 12 or a partial sequence thereof, for example. According to the sIgA-binding nucleic acid molecule of the present invention, it is possible to detect sIgA in saliva.

Secretory immunoglobulin a (sIgA)-binding nucleic acid molecule, sIgA analysis sensor, and sIgA analysis method

The present invention provides a novel nucleic acid molecule that can be used for detection of a wheat allergen. The wheat allergen-binding nucleic acid according to the present invention is characterized in that it binds to a wheat allergen with a dissociation constant of 20 nM or less, and preferably includes a polynucleotide consisting of either of base sequences of SEQ ID NOs: 1 and 2, for example.

Wheat allergen-binding nucleic acid molecule and use thereof

The present invention provides a novel nucleoside derivative or a salt thereof, a polynucleotide synthesis reagent, a method for producing a polynucleotide, a polynucleotide, and a method for producing a binding nucleic acid molecule. The nucleoside derivative or a salt thereof of the present invention is represented by the following chemical formula (1): where in the chemical formula (1), Su is an atomic group having a sugar skeleton at a nucleoside residue or an atomic group having a sugar phosphate skeleton at a nucleotide residue, and may or may not have a protecting group, Land Lare each independently a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms, Xand Xare each independently an imino group (&#x2014;NR&#x2014;), an ether group (&#x2014;O&#x2014;), or a thioether group (&#x2014;S&#x2014;), and the Ris a hydrogen atom or a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms.

The present invention provides a novel nucleic acid molecule that can be used for detection of &#x3b1;-amylase. The &#x3b1;-amylase-binding nucleic acid molecule of the present invention is characterized in that it binds to &#x3b1;-amylase with a dissociation constant of 17 nM or less, and preferably includes a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 22, for example. According to the nucleic acid molecule of the present invention, it is possible to detect &#x3b1;-amylase in saliva.

&#x3b1;-amylase-binding nucleic acid molecule and use thereof

The present invention provides a novel technique by which the redox activity of a nucleic acid molecule can be evaluated. An evaluation method of the present invention includes: a detection step of electrochemically detecting a redox reaction to a substrate, the redox reaction being catalyzed by a nucleic acid molecule to be evaluated, using a device that electrochemically detects a redox reaction; and an evaluation step of evaluating redox activity of the nucleic acid molecule from a result of the detection of the redox reaction. As the device, a device in which a base provided with a detection portion is included, the detection portion includes an electrode system, and the nucleic acid molecule to be evaluated is arranged on the base is used. In the present invention, it is preferred that a plurality of kinds of nucleic acid molecule to be evaluated is arranged on the base, and the plurality of kinds of nucleic acid molecules to be evaluated is evaluated by a single device.

Method for evaluating redox activity of nucleic acid molecule and nucleic acid molecule having redox activity

The present invention is intended to provide a novel fluorescence sensor for target analysis, a kit for target analysis, and a target analysis method using the same. The fluorescence sensor for target analysis according to the present invention includes a nucleic acid molecule that includes a G-quartet-forming nucleic acid region (D) that forms a G-quartet and a binding nucleic acid region (A) that binds to a target. In the absence of a target, formation of a G-quartet in the G-quartet-forming nucleic acid region (D) is inhibited. In the presence of a target, the target comes into contact with the binding nucleic acid region (A), the G-quartet is formed in the G-quartet-forming nucleic acid region (D) due to the contact, the G-quartet-forming region (D) and porphyrin forms a complex, and the complex generates fluorescence.

Fluorescence sensor for target analysis, kit for target analysis, and target analysis method using same

The present invention provides a novel sensor for detecting streptavidin (SA). The nucleic acid sensor for analyzing SA of the present invention includes the following nucleic acid element that includes a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to SA. The nucleic acid element is a double-stranded nucleic acid element including a first strand and a second strand. The first strand (ss1) includes the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order. The second strand (ss2) includes a stem-forming sequence (S), a loop-forming sequence (L2), and a stem-forming sequence (S) linked in this order. In this nucleic acid element, in the absence of SA, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (S) and (S), and in the presence of SA, the stem formation is released by a binding of the binding nucleic acid molecule (A) with the SA, and the catalytic function of the catalyst nucleic acid molecule (D) is exerted.

Device and method for analyzing streptavidin

The present invention provides a novel sensor for detecting a target. The nucleic acid sensor of the present invention includes a nucleic acid element that includes a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to a target. The nucleic acid element is a double-stranded nucleic acid element including a first strand and a second strand. The first strand (ss1) includes the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order. The second strand (ss2) includes a stem-forming sequence (S), a loop-forming sequence (L2), and a stem-forming sequence (S) linked in this order. In this nucleic acid element, in the absence of a target, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (S) and (S), and in the presence of ATP/target, the stem formation is released by a binding of the binding nucleic acid molecule (A) with the target, and the catalytic function is exerted.

Device and method for analyzing target

The present invention provides a novel nucleic acid element candidate molecule for use in screening for a nucleic acid element for target analysis and a novel screening method for screening for a nucleic acid element for target analysis using the same. The candidate molecule according to the present invention is a molecule for screening for a nucleic acid element for target analysis, being the following single-stranded nucleic acid (I): (I) a single-stranded nucleic acid comprising a catalyst sequence (D), a blocking sequence (B), and a binding sequence (A) that binds to a target, linked in this order. The blocking sequence (B) is complementary to a partial region (Dp) in the catalyst sequence (D). A terminal region (Ab) on the blocking sequence (B) side in the binding sequence (A) is complementary to a flanking region (Df) of the partial region (Dp) in the catalyst sequence (D) and is complementary to a terminal region (Af) on the side opposite to the blocking sequence (B) side in the binding sequence (A).

Nucleic acid element candidate molecule and screening method for screening for nucleic acid element for target analysis using the same

The present invention provides a novel nucleic acid molecule that can be used for detection of peanuts. The peanut-binding nucleic acid molecule of the present invention is characterized in that it binds to a peanut allergen with a dissociation constant of 10 nM or less, and preferably contains a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 15, for example. It is also preferable that, for example, the peanut-binding nucleic acid molecule of the present invention binds with significant specificity to the peanut allergen rather than to a soybean protein.

Peanut-binding nucleic acid molecule and use thereof

A nucleic acid molecule utilizable fordetection is provided. The nucleic acid molecule which binds toincludes any of the following polynucleotides (a) to (d): (a) a polynucleotide composed of any of base sequences of SEQ ID NOs: 1 to 17; (b) a polynucleotide composed of a base sequence obtained by deletion, substitution, insertion, and/or addition of one or more bases in any of the base sequences in the polynucleotide (a) and is bound to; (c) a polynucleotide composed of a base sequence having an identity of 80% or more to any of the base sequences in the polynucleotide (a) and is bound to; and (d) a polynucleotide composed of a base sequence complementary to a polynucleotide which hybridizes to the polynucleotide (a) composed of any of the base sequences under stringent conditions and is bound to

[object Object]

The technique by which simple analysis of an intended subject to be analyzed can be carried out is provided. In this technique, a nucleic acid elementfor use in analysis including: a first nucleic acid part; and a second nucleic acid partis used. In the nucleic acid element, the first nucleic acid partis a binding part that can bind to a subjectto be analyzed, and the second nucleic acid partis a labeling part that can distinguish between binding and non-binding of the first nucleic acid partto the subject. It is preferred that the first nucleic acid partis an aptamer against the subject. The subjectcan be analyzed easily by using the nucleic acid element, binding the subjectto the first nucleic acid part, and then analyzing the binding with the second nucleic acid part

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Tokyo, Japan

Jou Akitomi