The United States of America, As Represented by the Secretary, Department of Health and Human Services

Jothikumar Narayanan

Vincent Hill

Brian Holloway

Prithiviraj Jothikumar

This disclosure related to methods and reagents for isothermal amplification of nucleic acid molecules. In some embodiments, methods are provided for amplification of a nucleic acid molecule from a biological sample. Additional embodiments include identification of a target nucleic acid molecule in a biological sample using the disclosed amplification methods.

Methods and reagents for amplifying nucleic acids

This application provides photoinduced electron transfer (PET) nucleic acid molecules that can be used detect and amplify nucleic acid molecules, such as target nucleic acid molecules. These PET tags can be attached to the 5'-end of a target sequence-specific primer, thereby generating a PET primer. In particular examples, a PET tag includes a 5&#x2032;-labeled nucleotide that can be quenched by at least two consecutive Gs within the tag sequence, and is unquenched when the PET tag hybridizes with its complementary nucleic acid molecule. Also disclosed are methods of using PET primers in nucleic acid amplification, such as real-time PCR.

Photoinduced electron transfer (PET) primer for nucleic acid amplification

This application provides universal labeled primers for detection and amplification of nucleic acid molecules. These universal primers can be attached to the 5'-end of a target sequence-specific primer. In particular examples, the universal primer includes a labeled nucleotide flanked on both sides a nucleotide whose complement nucleotides changes a detectable signal from the label when the universal primer hybridizes with its complementary nucleic acid molecule. Also disclosed are methods of using the universal primer in nucleic acid amplification, such as real-time PCR.

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Atlanta, GA, United States of America

Jothikumar Narayanan