Axis-shield Asa

Abbott Rapid Diagnostics International Unlimited Company

Jostein Holtlund

Stig Morten Borch

Jan Roger Karlson

Inger Lise Lauvstad

Thorstein Seim

Tore Janson

Hege Tøn

Andrew Thomas Campbell

Morten Borch

Ellen Dworsky

Lise Corneliussen

Hege Ton

Kerstin Bernstrom

Hege Toen

Provided herein is technology relating to methods for assaying a cell-containing body sample and particularly, but not exclusively, to treating a sample under conditions to cause cell lysis, preferably by means of a detergent; and subjecting the lysed sample to conditions causing the cleavage of nucleic acid molecules. The technology additionally relates to using nucleic acid cleavage conditions to enhance a membrane assay, a device for carrying out such an assay, and a kit for use in the assay.

Membrane assay method

The present invention provides an assay method for a cell-containing body sample, the method comprising treating the sample under conditions whereby to cause cell lysis, preferably by means of a detergent; and subjecting the thus-generated lysed sample to conditions causing the cleavage of nucleic acid molecules. The invention additionally provides the use of nucleic acid cleavage conditions in enhancing a membrane assay, a device for carrying out such an assay, and a kit for use in the assay.

An assay apparatus comprising: i) an assay cartridge () comprising at least one well (-) and a pipette () positionable in at least one said well; ii) a holder arranged to received said cartridge; iii) drive means operable to position said pipette in selected wells of said cartridge; iv) a gas pressure applicator couplable to said pipette whereby to cause liquid flow through said membrane; and v) a radiation detector operable to detect radiation from a well of said cartridge of said cartridge or from said pipette.

Assay system

An assay apparatus comprising: i) an assay cartridge () comprising at least one well (-) and a pipette () positionable in at least one said well; ii) a holder arranged to receive said cartridge; iii) drive means operable to position said pipette in selected wells of said cartridge; iv) a gas pressure applicator couplable to said pipette whereby to cause liquid flow through said membrane; and v) a radiation detector operable to detect radiation from a well of said cartridge or from said pipette.

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Oslo, Norway

Jostein Holtlund