Johns Hopkins University

Joshua T Wolfe

Jared D Evans

David A Weitz

Marcia W Patchan

Andrew B Feldman

Jennifer L Sample

Jason J Benkoski

Luis Garza

Jeffrey S Lin

Xiomara Calderon-Colon

Julia B Patrone

Jennifer L Breidenich

Andrew S Pekosz

John Heyman

Lisa A Kelly

Amanda Nelson

David K Karig

Huong Le

James C Crookston

Peter M Thielen

Thomas S Mehoke

Mellisa L Theodore

Sewon Kang

A topical composition includes a nanoemulsion of a plurality of hydrophobic particles having a hydrophilic coating therein. The hydrophobic particles are derived from the same or different hydrophobic material and each hydrophobic particle has a melting point below the melting point of the respective hydrophobic material. The hydrophobic particles comprise a mean particle size of less than about 10 nm, and the nanoemulsion further includes one or more pharmaceutically active agents.

Topical compositions and methods of detection and treatment

A method of directed evolution screening includes selecting a protein expression platform for an evolution target, expressing a key rupture gene configured to trigger droplet rupture, developing gene regulatory circuits to control expression of the key rupture gene as a function of performance of the evolution target, encapsulating expression components in droplets, and triggering droplet rupture by expressing a rupture agent from the key rupture gene.

Programmed droplet rupture for directed evolution

A method for synthesizing an antiserum for rapid-turnaround therapies includes collecting antibody-secreting cells from a test subject, wherein the test subject has been exposed to a target biological agent and has produced an antibody response; selecting a subset of the antibody-secreting cells, the subset of the antibody-secreting cells producing antibodies that neutralize the target biological agent; generating variable-region-coding DNA sequences from the antibodies that neutralize the target biological agent; tagging amplicons of the variable-region-coding DNA sequences with unique nucleic acid identifiers to associate the variable-region-coding DNA sequences derived from individual ones of the subset of the antibody-secreting cells; analyzing antibody-type distribution in a natural immune response; synthesizing antibodies from the variable-region-coding DNA sequences to form synthetic antibodies; and mixing the synthetic antibodies in a proportion equal to the antibody-type distribution in the natural immune response to form the antiserum.

Synthetic antiserum for rapid-turnaround therapies

A method for limited input whole genome sequencing of RNA viruses includes isolating a viral RNA sample, converting the viral RNA sample to a double-stranded viral cDNA sample, constructing a double-stranded viral cDNA amplicon library from the double-stranded viral cDNA sample, and sequencing the double-stranded viral cDNA amplicon library to obtain a double-stranded viral cDNA sample sequencing read.

Efficient deep sequencing and rapid genomic speciation of RNA viruses (vRNAseq)

Growing community of inventors

Bethesda, MD, United States of America

Joshua T Wolfe