Lawrence Livermore National Security, LLC

Lawrence Livermore National Laboratory, LLC

Joshua R Deotte

Eric B Duoss

Christopher M Spadaccini

Sarah Elyse Baker

Nikola Dudukovic

Fang Qian

William J Benett

Neil Reginald Beer

Xiaoyu Zheng

Jennifer Marie Knipe

James M Frank

Samantha Ruelas

Roger D Aines

Joshuah K Stolaroff

Cheng Zhu

Scott McCall

Bryan D Moran

Alexander A Baker

Julie A Mancini

Seth Evan Watts

Alexandra Golobic

James T Cahill

Jeremy Taylor Feaster

Erika Jo Fong

Jonathan Tesner Davis

Maria C Prieto Conaway

Michael John Troksa

Hawi Bacha Gemeda

Karen Ruth Dubbin

Bio-ink comprising freeze-dried cells, methods of making a living structure from a bio-ink material of freeze-dried cells, and methods of using the living structure for biosensing, tissue regeneration, environment sensing, drug discovery, catalysis, and/or clinical implementation are described herein.

Bio-ink structures and methods of producing the same

The present disclosure relates to an additive manufacturing system. The system incorporates a nozzle in communication with a quantity of feedstock material including granular metal powder particles. The nozzle is configured to deposit the granular metal powder particles on at least one of a build plate or a previously formed material layer, to form at least one layer of a part. The system also incorporates a magnetic field generator subsystem for producing a magnetic field configured to orient the granular metal powder particles in a desired orientation before fusing of the granular metal powder particles occurs.

System and method for multimaterial powder bed patterning for use in additive manufacturing

The present disclosure relates to an engineered, additively manufactured, microfluidic cellular structure formed from a plurality of cells, wherein the cells are each formed from a plurality of interconnected elements. The cells have voids and each cell is open at upper ends thereof. The cells each communicate at a point below its upper end with a common channel. The cells are each configured to accept a fluid and operate to channel the fluid into the common channel and to hold the fluid received therein for later selective withdrawal from the structure.

Systems and methods for reaction and transport engineering via cellular fluidics

The present disclosure relates to an additive manufacturing system. In one embodiment the system makes use of a reservoir for holding a granular material feedstock. A nozzle is in communication with the reservoir for releasing the granular material feedstock in a controlled fashion from the reservoir to form at least one layer of a part. An excitation source is included for applying a signal which induces a controlled release of the granular material feedstock from the nozzle as needed, to pattern the granular material feedstock as necessary to form a layer of the part.

Multimaterial powder bed patterning for additive manufacturing method

The present disclosure relates to a lattice substrate adapted for use in direct ionization mass spectrometry. The substrate may have a plurality of tessellated unit cells forming an integral structure. Each tessellated unit cell may have a dimension of no more than about 1.5 mm and may include a plurality of pores arranged in an ordered pattern. The substrate may further include a form factor suitable for use with a direct ionization mass spectrometry system.

Systems and methods lattice spray substrates for mass spectrometry

A functionally graded material is formed by pipetting individual micro-or-nano-litter droplets with a variety of materials including multi-nanostructured material (nanowires, carbon nanotubes, enzymes, multi-element and/or multi-color, multi-biomolecules) and UV polymerization of the flat hydrogel meniscus surface formed at the carrier fluid interface. After step-by-step droplet pipetting and subsequent layer-by-layer UV polymerization via a digital mask, the complete fabricated part without supporting layers is taken out of the carrier fluid while the un-cured micro-litter residue is conveniently suctioned out of the carrier fluid.

Emulsion stereolithography and 3D printing of multimaterials and nanoscale material gradients

An engineered unit cell is disclosed for flowing a fluid therethrough in three dimensions. The unit cell may have a substrate with a plurality of flow channels around and between struts formed within the substrate. The struts may each be formed with a desired shape and orientation within the substrate to achieve a desired degree of fluid flow through the flow channels, in each of one of three dimensions, through the unit cell.

Lattice microfluidics

A system for building a structure. The system includes a reservoir; an immiscible fluid in the reservoir; a membrane operatively connected to the reservoir; channels and delivery ports in the membrane; a microliter amount of curable resin; a delivery system for delivering the microliter amount of curable resin to channels and delivery ports in the membrane and to the reservoir in contact with the immiscible fluid in the reservoir; and an energy source adapted to deliver energy to the reservoir onto the microliter amount of curable resin for building the structure.

Microporous membrane for stereolithography resin delivery

An additive manufacturing system utilizing an oxygen-permeable membrane to facilitate low-volume printing and high-speed resin change in a stereolithography-based 3D printer. The membrane is used to constrain the fluid surface at the focal plane of the stereolithography printer and a carrier fluid is used to hold the fluid against the membrane. The small amount of resin is quickly switched using integrated microfluidic channels.

Additive manufacturing with integrated microliter resin delivery

The rapid thermal cycling of a material is targeted. A microfluidic heat exchanger with an internal porous medium is coupled to tanks containing cold fluid and hot fluid. Fluid flows alternately from the cold tank and the hot tank into the porous medium, cooling and heating samples contained in the microfluidic heat exchanger's sample wells. A valve may be coupled to the tanks and a pump, and switching the position of the valve may switch the source and direction of fluid flowing through the porous medium. A controller may control the switching of valve positions based on the temperature of the samples and determined temperature thresholds. A sample tray for containing samples to be thermally cycled may be used in conjunction with the thermal cycling system. A surface or internal electrical heater may aid in heating the samples, or may replace the necessity for the hot tank.

Methods and compositions for rapid thermal cycling

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Livermore, CA, United States of America

Joshua R Deotte