Emulate, Inc.

Lightlab Imaging, Inc.

Sage Science, Inc.

Other

Joshua Gomes

Christopher David Hinojosa

Daniel Levner

Josiah Daniel Sliz

Kyung Jin Jang

Guy Robert Thompson, Ii

Jose Fernandez-Alcon

Jacob Freake

Doug Sabin

Desmond Christopher Adler

Douglas Grosvenor Sabin

David Winston

Susan Moynihan

Norman Wen

T Christian Boles

Matthew Daniel Solomon

Charles Sidoti

Todd J Barbera

Petrus Wilhelmus Martinus Van Ruijven

Christian Alexander Potzner

Patrick Sean Tuohy

Simranjit Singh

Guy Robert Thompson

Paul Sabin

Craig Henshaw

Lewis Rowe

Timothy F Deso

David James Coon

Christian T Boles

Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.

Removing bubbles in a microfluidic device

A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

Controlling pressure

A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.

Pressure manifold and culture module

The devices, methods and systems are described for providing controlled amounts of gas, gas pressure and vacuum to microfluidic devices the culturing of cells under flow conditions. The devices, methods, and systems contemplated here may also be used to control the environment surrounding the microfluidic devices; offer user control over experiments comprising microfluidic devices, such as the ability to directly or remotely control experiment conditions; and comprise information aggregation and transmission, such that experimental data may be collected, stored, aggregated and transmitted to users.

Gas mixer and pressure apparatus

Perfusion module lid without ports

The invention relates to a perfusion manifold assembly that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with an assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

Perfusion manifold assembly

Growing community of inventors

Somerville, MA, United States of America

Joshua Gomes