10X Genomics, Inc.

Joshua Delaney

Shalini Gohil

Adam Lowe

Christopher Hindson

Andrew D Price

Meiliana Tjandra

Albert Dale Kim

Joseph Francis Shuga

Yi Luo

Monica Nagendran

Jill Herschleb

Veronica Emelina Gonzalez Muñoz

Joshua Gu

Tathagata Mukherjee

James Francis Perna, Iii

Kristen Pham

The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.

Catalytic de-crosslinking of samples for in situ analysis

The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

Methods for generating barcoded nucleic acid molecules using fixed cells

Compositions and methods for using fixed biological samples

The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

Compositions, methods, and systems for bead formation using improved polymers

Growing community of inventors

Oakland, CA, United States of America

Joshua Delaney