Tune Therapeutics, Inc.

Duke University

Joshua B Black

Kendra Congdon

Veronica Gough

Jason Dean

Brian Cosgrove

Britta Jones

Charles A Gersbach

Isaac B Hilton

David G Ousterout

Pablo Perez-Pinera

Pratiksha I Thakore

Ami M Kabadi

Provided herein are epigenetic-modifying DNA-targeting systems, such as CRISPR-Cas/guide RNA (gRNA) systems, for the transcriptional repression of Hepatitis B viral (HBV) genes to promote a cellular phenotype that leads to the reduction of HBV infection. In some embodiments, the epigenetic-modifying DNA-targeting systems bind to or target a target site of at least one gene or regulatory element thereof in a Hepatitis B viral DNA sequence in cell. In some aspects, the provided systems relate to the transcriptional repression of one or more Hepatitis B viral gene and/or regulatory element thereof. In some aspects, also provided herein are methods and uses related to the provided compositions, for example in repressing Hepatitis B viral replication and expression in connection with Hepatitis B infections.

Compositions, systems, and methods for regulation of Hepatitis B virus through targeted gene repression

Compositions, systems, and methods for regulation of Hepatitis b virus through targeted gene repression

Compositions, systems, and methods for regulation of hepatitis B virus through targeted gene repression

Compositions, systems, and methods for regulation of hepatitis b virus through targeted gene repression

Disclosed herein are Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) 9-based system related compositions and methods of using said CRISPR/Cas9-based system related compositions for altering gene expression and genome engineering. Also disclosed herein are compositions and methods of using said compositions for altering gene expression and genome engineering in muscle, such as skeletal muscle and cardiac muscle.

RNA-guided gene editing and gene regulation

Growing community of inventors

Durham, NC, United States of America

Joshua B Black