Amyris, Inc.

Joshua Adam Lerman

Hanxiao Jiang

Penelope R Chua

Annie Ening Tsong

Michael Leavell

Jessica Walter

Thomas Jon Scherbart

Benjamin Yap

Chandresh Thakker

Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.

Methods for decoupling yield and productivity of a non-catabolic compound produced by a host cell

Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides. The yeast cells include one or more heterologous nucleic acids that encode enzymes of a human milk oligosaccharide biosynthetic pathway. The yeast cells do not include a heterologous nucleic acid encoding a fucokinase. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering human milk oligosaccharides generated by the yeast cells.

Biosynthesis of compounds in yeast

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Emeryville, CA, United States of America

Joshua Adam Lerman