Case Western Reserve University

Uh Cleveland Medical Center

The United States Government as Represente

Joseph Willis

Sanford D Markowitz

Amitabh Chak

Rom Leidner

Dennis Siedlak

Dean J Secrest

Jeffrey Silver Taggart

Helen Moinova

Thomas LaFramboise

Anant Madabhushi

Michael P Boutillette

Rebecca L Blice

Richard Yazbeck

Lishan Aklog

Rebecca Blice

Dawn Dawson

Neeraj Kumar

Sanford Markowitz

Dean Secrest

A device for collecting a biological sample in a patient includes a collection portion having a first axial end portion and a second axial end portion. The second axial end portion has a collapsed position and an expanded position. The second axial end portion moves in an axial direction relative to the first axial end portion when the second axial end portion moves between the collapsed position and the expanded position. The second axial end portion extends axially into the first axial end portion and has a concave shape when in the collapsed position. The second axial end portion is convex when in the expanded position.

Device for collecting a biological sample

A device for collecting a biological sample in a subject, the device including an inflatable portion attached to a tubular member, and designed to expand from within the tubular member when inflated and to retract within the tubular member, a surface on the inflatable portion provided with varied thickness to facilitate movement of the inflatable portion between an expanded state and a retracted state, and a plurality of projections on the surface to allow collection of the biological sample when the inflatable portion is in the expanded state.

Device for biological cell collection and method of use

The disclosure provides methods for identifying genomic loci (e.g., vimentin and/or SqBE18) that are differentially methylated in metaplasias (e.g., Barrett's esophagus) and/or neoplastic cancers (e.g., esophageal cancers). Identification of methylated genomic loci has numerous uses, including for example, to characterize disease risk, to predict responsiveness to therapy, to non-invasively diagnose subjects and to treat subjects determined to have gastrointestinal metaplasias and/or neoplasias.

Methods and compositions for detecting esophageal neoplasias and/or metaplasias in the esophagus

Embodiments discussed herein facilitate determination of cancer stages based at least in part on shape, size, and/or texture features of cancer nuclei. One example embodiment is a method, comprising: accessing at least a portion of a digital whole slide image (WSI) comprising a tumor; segmenting (e.g., via a first deep learning model) the tumor on the at least the portion of the digital WSI; segmenting (e.g., via a second deep learning model) cancer nuclei in the segmented tumor; extracting one or more features from the segmented cancer nuclei; providing the one or more features extracted from the segmented cancer nuclei to a trained machine learning model; and receiving, from the machine learning model, an indication of a cancer stage of the tumor.

Distinguishing colon cancer stages based on computationally derived morphological features of cancer nuclei

This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as neoplasia of the upper or lower gastrointestinal tract, pancreas, and/or bladder.

Methods and compositions for detecting gastrointestinal and other cancers

This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as neoplasia of the upper or lower gastrointestinal tract, pancreas, and/or bladder. Nucleic acid sequences for regions of vimentin that are found to be differentially methylated are described. The present disclosure further describes bisulfite-converted vimentin template DNA sequences. Oligonucleotide primer sequences for use in assays (e.g., methylation-sensitive PCR assays or HpaII assays) designed to detect the methylation status of the vimentin gene are also described.

This disclosure provides methods and compositions for inhibiting immune responses. The disclosure also provides methods and compositions for inhibiting graft rejection and promoting or prolonging graft survival.

Methods for diagnosing and treating cancers

Growing community of inventors

Shaker Heights, OH, United States of America

Joseph Willis