Akzo Nobel N.v.

Joseph W Romano

Roxanne Shurtliff

Eun Mi Lee

Kimberly G Williams

Ranajit Pal

Gregory J Hurteau

An isothermal transcription based amplification assay for dengue virus RNA uses primer combinations for sequences within the envelope gene or the 3&prime; non-coding region of the virus and a probe. Probes may be specific for a serotype of dengue virus.

Isothermal transcription based assay for the detection and genotyping of dengue virus

An isothermal transcription based amplification assay for the detection or quantification of chemokine RNA uses primers and probes for sequences within the gene for RANTES, MIP-1&agr; and MIP-1 &bgr;. The quantitative system uses an internal control Q of a mutant version of each gene. Target specific primers and probes are also disclosed.

Isothermal transcription based assay for the detection and quantification of chemokines rantes, MIP-1&agr; and MIP-1&bgr;

A method and a test kit for genotyping the CCR5 chemokine receptor. CCR5 transcript DNA is amplified using transcription based amplification. The amplification product is detected through the hybridization of appropriately labeled oligonucleotide wild type and mutant probes. In a liquid hybridization based ECL analysis, the mutant probe is a 16mer having the sequence 5&prime;TCCATACATTAAAGAT3&prime; &lsqb;SEQ ID NO:9&rsqb;. The method and kit can be used to predict.

CCR5 RNA transcription based amplification assay

An isothermal transcription based amplification assay for the detection or quantification of chemokine RNA uses primers and probes for sequences within the gene for RANTES, MIP-l.alpha. and MIP-1 .beta.. The quantitative system uses an internal control Q of a mutant version of each gene. Target specific primers and probes are also disclosed.

Isothermal transcription based assay for the detection and

An isothermal transcription based amplification assay for MDC RNA uses primer combinations for sequences within the MDC gene. A quantitative control uses a mutant RNA for comparison.

Isothermal transcription based amplification assay for the detection and

An isothermal transcription based amplification assay for the detection of HTLV I RNA uses primers and probes for sequences within the tax gene, the pol gene, or the gag gene. An isothermal transcription based amplification assay for the detection of HTLV II uses primers and probes for sequences within the tax gene. Target specific primers and probes are also disclosed.

Isothermal transcription based assay for the detection of HTLV I and

An isothermal transcription based amplification assay for dengue virus RNA uses primer combinations for sequences within the envelope gene or the 3' non-coding region of the virus and a probe. Probes may be specific for a serotype of dengue virus.

Isothermal transcription based assay for the detection and genotyping of

A method for determining virus replication in human cells by human retrovirus using RNA amplification comprising detecting the hybridization of an RNA probe which specifically hybridizes with spliced RNA and not with genomic RNA. This method permits early detection of RNA replication resulting from primary infection without detecting non-replicating virus.

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Derwood, MD, United States of America

Joseph W Romano