Hoffmann-la Roche Inc.

The Perkin-elmer Corporation

Joseph T Widunas

Kary B Mullis

Richard A Leath

Louis M Mezei

Larry Johnson

Timothy J Wennberg

There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.

System for automated performance of the polymerase chain reaction

Apparatus and method for performing automated amplification of nucleic

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Freemont, CA, United States of America

Joseph T Widunas