Amersham Life Science, Inc.

Amersham Biosciences Corp

Joseph Szasz

Maria Davis

Carl Williams Fuller

Frank T Robb

Kimberly M Borges

Thermostable DNA polymerases both in native form and having single amino acid substitutions and optionally N-terminal deletions are disclosed. These polymerases exhibit a substantial improvement of DNA sequencing performance compared to Taq DNA polymerase. The instant DNA polymerases also possess improved salt tolerance.

DNA polymerases having amino acid substitutions and homologs thereof

Enzymatically active thermostable alkaline phosphatases from Rhodothermus marinus, Thermus thermophilus, and Thermosipho africanus.

Thermostable alkaline phosphatases

Alkaline phosphatase from Thermus thermophilus has been isolated. The enzyme has a pH optimum of greater than 10.5 and is stable to heating at 65.degree. C for 1 hour. The claimed invention relates to a method of detecting nucleic acids in a sample by providing a nucleic acid probe labelled with thermostable alkaline phosphatase from Thermus thermophilus; contacting the sample with said labelled nucleic acid; and detecting said nucleic acid in said sample by means of said thermostable alkaline phosphatase.

Thermostable alkaline phosphatase of thermus thermophilus

Purified nucleic acid comprising a nucleotide base sequence selected from sequence ID NOS.: 1-8.

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Chardon, OH, United States of America

Joseph Szasz