Roche Molecular Systems, Inc.

Other

Roche Diagnostics Gmbh

F. Hoffmann-La Roche Ag

Joseph San Filippo

Thomas W Myers

Keith Bauer

Fred Lawrence Reichert

Nancy J Schoenbrunner

Shawn Lee Suko

Rachel Shahinian

Ellen H Fiss

Dirk Zimmermann

Nicolas Newton

Hermann Leying

Eberhard Russmann

Xiaoning Wu

Karen K Y Young

Heike Wilts

Shawn Suko

Keith A Bauer

Fred L Reichert

Karen Kwok Ying Young

Nancy Schoenbrunner

Fred Reichert

Disclosed are mutant DNA polymerases having increased 3'-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

DNA polymerases with increased 3'-mismatch discrimination

A method of simultaneously amplifying genotypes 1, 2, 3 and/or 4 of HEV is disclosed comprising amplifying the genotypes 1, 2, 3 and/or 4 of HEV with one single none-degenerate forward primer partially overlapping the 5'UTR region of HEV and at least one reverse primer. Also disclosed are related methods comprising a probe, and kits for the detection of genotypes 1, 2, 3 and/or 4 of HEV.

HEV Assay

The invention comprises a reversible oligonucleotide inhibitor of nucleic acid polymerases. Methods of designing said inhibitors and using said inhibitors in amplification and detection of nucleic acid, particularly detection of RNA by RT-PCR are also disclosed.

Oligonucleotide inhibitor of DNA polymerases

Disclosed are DNA polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of RT and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

DNA polymerases with improved activity

HEV assay

Methods for conducting primer extension using DNA polymerases with improved activity

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Dublin, CA, United States of America

Joseph San Filippo