Leland Stanford Junior University

Screen Holdings Co., Ltd.

Joseph Russell Landry

Olav Solgaard

Michael J Mandella

Stephen S Hamann

Ryosuke Itoh

An axially swept light sheet fluorescence microscope has illumination optics capable scanning the focus region of a line beam along an illumination optical axis to illuminate a light sheet in a sample plane, and detection optics capable of collecting fluorescence light from the sample plane and imaging the collected light on a light detector with a rolling shutter. A microcontroller synchronizes the rolling shutter with the scanning of the focus region. The illumination optics performs the axial scanning using a linear phased array of independently controllable electrostatically driven optical elements controlled by the microcontroller.

Rapid axial scanning for light sheet microscopy using a phased array

A system, including a structured illumination stage to provide a spatially modulated imaging field is provided. The system further includes a spatial frequency modulation stage to adjust the frequency of the spatially modulated imaging field, a sample interface stage to direct the spatially modulated imaging field to a sample, and a sensor configured to receive a plurality of fluorescence emission signals from the sample. The system also includes a processor configured to reduce a sample scattering signal and to provide a fluorescence emission signal from a portion of the sample including the spatially modulated imaging field. A method for using the above system to form an image of the sample is also provided.

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Stanford, CA, United States of America

Joseph Russell Landry