Other

University System of Maryland

Becton Dickinson and Company Limited

Joseph R Lakowicz

Chris D Geddes

Henryk Szmacinski

Ramachandram Badugu

Ignacy Gryczynski

Zygmunt Gryczynski

Klaus W Berndt

Govind Rao

Badri P Maliwal

Felix N Castellano

Zakir S Murtaza

Richard B Thompson

Ewald Alois Terpetschnig

Leah Tolosa

Robert A Hoffman

Thomas G Burke

Henryk Malak

Alvydas J Ozinskas

E Albert Reece

Michael Lynn Johnson

Leslie W J Baillie

Bertram G Pinsky

Henry K Szmacinski

Qing Chang

Omoefe Abugo

Candace McCombs

Mustafa Habib Chowdhury

Lisa Eichhorn

Chandran R Sabanayagam

Peter A Koen

Engin U Akkaya

Nicolas Dicesare

Kazimierz Nowaczyk

Leslie Baillie

Les Baillie

A material, article, system and method include a probe composition that includes a hydrophobic portion, a hydrophilic portion, an analyte-binding portion and a fluorophore portion. The analyte-binding portion is configured to bind to an analyte in an aqueous solution. The fluorophore portion is configured to change an optical property of fluorescent light emitted in response to incident excitation light when the probe composition changes between a first state in which the analyte is not bound to the analyte-binding portion and a second state in which the analyte binds to the analyte-binding portion. A material includes the probe composition and a silicone hydrogel substrate having a hydrogel network that allows flow of aqueous solution through the solution and a silicone network that occupies interstices of the hydrogel network. A contact lens having the material enables remote detection of glucose concentration in tear fluid of a subject.

Silicone hydrogel based fluorescent assay and contact lens

Techniques for metal enhanced fluorescence include determining a calibration curve that relates concentration of a particular analyte to at least one of intensity or lifetime of fluorescent emissions at a plasmonic substrate in response to incident light, for a plurality of known concentrations of the particular analyte mixed with a reagent. The reagent comprises a detection molecule. A concentration of the particular analyte in a vicinity of a cell in a sample is determined directly from the calibration curve and measurements, in response to the incident light, of at least one of intensity or lifetime of fluorescent emissions at the plasmonic substrate in contact with the cell and reagent.

Plasmonic substrates for metal-enhanced fluorescence based sensing, imaging and assays for cells

Techniques for enhanced fluorescence include a functionalized substrate for a target optical frequency comprising a one dimensional photonic crystal that is functionalized with a bioactive target molecule that has an affinity for a particular analytic. The one dimensional photonic crystal includes a plurality of dielectric layers including a plurality of high index of refraction layers alternating with a plurality of low index of refraction layers. The thickness of each layer is within a factor of four of a wavelength of the optical frequency in the layer. For emissions from a fluorophore bound to the target molecule and excited by incident light, there is an emission intensity maximum centered at an angle independent of the direction of the incident light.

One dimensional photonic crystals for enhanced fluorescence based sensing, imaging and assays

Techniques for enhanced fluorescence include a Tamm substrate for a target optical frequency comprising a metal nanoscale layer deposited on a Bragg grating. The Bragg grating includes multiple dielectric layers including multiple high index of refraction layers alternating with multiple low index of refraction layers. The dielectric layers are parallel to the metal nanoscale layer; and, the thickness of each dielectric layer is about a fourth of a wavelength of the target optical frequency in the layer. The metal nanoscale layer is configured to host a fluorophore such that an S polarized emission from the fluorophore at the target optical frequency propagates out of the substrate perpendicular to the plurality of dielectric layers.

Tamm structures for enhanced fluorescence based sensing, imaging and assays

The present invention relates to an assay including a surface having silver colloids or islands attached thereto. Attached to the surface and/or silver colloids/islands are polynucleotides which are complimentary to a target polynucleotide sequence. The assay is performed by adding the target polynucleotide sequence to the assay surface and allowing it to hybridize with the capture polynucleotides. Fluorophore-labeled capture polynucleotides are added and hybridize to the target polynucleotide. Bound target polynucleotides are detected by metal enhanced fluorescence.

High-sensitivity assays for pathogen detection using metal-enhanced fluorescence

Quaternary nitrogen heterocyclic boronic acid-containing compounds are described, which are sensitive to glucose and fructose, as well as a variety of other physiologically important analytes, such as aqueous chloride and iodide, and a method of using the compounds. Also disclosed is a contact lens doped with the quaternary nitrogen heterocyclic boronic acid-containing compound, and a method of using the doped contact lens to measure the concentration of analyte in tears under physiological conditions.

Quaternary nitrogen heterocyclic compounds for detecting aqueous monosaccharides in physiological fluids

Provided herein are methods for imaging subwavelength structures in three dimensions and with high resolution. The methods comprise illuminating subwavelength structures with an illuminating wavelength of light and detecting the self-image generated thereby at a distance distal to the structures. Also provided is a method for confining propagating light to a sub-diffraction limit dimension by illuminating a surface of a metal structure with subwavelength features with a wavelength of coherent light such that light propagating from the features is confined to a dimension that is a sub-diffraction limit thereof.

Subwavelength resolution optical microscopy

A method and kit for determining the quantity of an analyte include providing a functionalized substrate and a reagent. The functionalized substrate includes metallic nanoparticles and a plurality of substantively identical bioactive target molecules affixed to a substrate. The bioactive target molecule binds to a particular analyte. The reagent includes identical detection molecules. Each detection molecule includes a fluorophore, and binds to a particular analyte or competes with a particular analyte for binding to the target molecule. The functionalized substrate is contacted to a test sample and the reagent. The functionalized substrate and a covering solution are exposed to polarized electromagnetic waves that excite the fluorophore. A quantity of the particular analyte in the test sample is determined based on measuring polarization anisotropy of fluorescent emissions from the substrate and the covering solution.

Metal-enhanced fluorescence for polarization-based affinity assays

Compositions and methods for increasing the fluorescence intensity of molecules are provided. In particular, compositions and methods directed to increasing the intrinsic fluorescence of biomolecules and low quantum yield fluorophores are described. The intrinsic fluorescence of biomolecules is increased by positioning a metal particle and a biomolecule at a distance apart sufficient to increase the radiative decay rate of the biomolecule. Methods for the identification of nucleic acids are also provided. The compositions and methods can also be used to increase the emission of any fluorophore, such as the extrinsic probes used to label biomolecules.

Radiative decay engineering

The present invention relates to a cyanide detection method using fluorescence and cyanide sensitive boronic acid containing fluorophores, wherein a change in a measured fluorescent property correlates to the concentration of the cyanide compound in a biological or environmental test sample.

Cyanide sensing compounds and uses thereof

The spectroscopic and photophysical properties of fluorescent probes comprising donor-acceptor derivatives comprising the boric acid group or a derivative of boric acid, B(OH)(or borate ion, BO(OH)), arsenious acid, HAsO(or arsenite ion, HAsO), telluric acid, HTeO(or tellurate ion, HTeO) or germanic acid, Ge(OH)(or germanate ion, GeO(OH)) are described. Method of using said probes are also provided.

Fluorescent probes for saccharrides

A new approach is described to making luminophores which display long emission wavelengths, long decay times, and high quantum yields. These luminophores are covalently linked or otherwise closely associated pairs with a long lifetime resonance energy transfer (RET) donor e.g., a ruthenium (Ru) metal-ligand complex, and a long wavelength acceptor, e.g., Texas Red. The donor and acceptor can be covalently linked by, e.g., poly-proline spacers. The long lifetime donor results in a long lived component in the acceptor decay which is due to RET. The quantum yield of the luminophores approaches that of the higher quantum yield acceptor, rather than the lower quantum yield typical of metal-ligand complexes. The emission maxima and decay time of such tandem luminophores can be readily adjusted by selection of the donor, acceptor and distance between them. Luminophores with these useful spectral properties can also be donor-acceptor pairs brought into close proximity by some biochemical association reaction. Luminophores with long wavelength emission and long lifetimes have numerous applications in biophysics, clinical diagnostics, DNA analysis and drug discovery.

Long wavelength long lifetime luminophores

The present invention is directed to optical structures and methods detecting the fluorescence of a molecule using metal-enhanced fluorescence. In particular, the invention describes the use of surface plasmon excitation for excitation of fluorophores near the metal surface and the efficient collection of the emission by coupling into the plasmon resonance and directing towards the detector. More particularly, the present invention makes use of the unique directionality of the plasmon induced fluorescence signal. The present invention is directed to optical structures using metal enhanced fluorescence including: an optical fiber having a conductive external coating; a light emitting diode (LED) having a conical shaped depression with curved sides on a front end surface, the curved sides having a conducting coating on the outer surface with respect to the LED.

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Ellicott City, MD, United States of America

Joseph R Lakowicz