Abbott Laboratories Corporation

Abbott Molecular Inc.

Abbott Point of Care Inc.

Joseph P Skinner

Qiaoqiao Ruan

Sergey Y Tetin

Sonal Sadaria Nana

Gordon Bruce Collier

Dan Wang

Frank Pawlowski

Susan Gayda

Mark A Talmer

Barry Lee Dowell

Timothy J Patno

Daniel J Harris

Sylvia C Saldana

Jason E Bryant

Jack H Kessler

Brett W Johnson

David M Peters

Daniel P Casazza

Eric Yeaton

Timothy P Henning

Chadwick M Dunn

Wesley W Addison, Ii

Jody L Keck

Austin Lines

Jack Kessler

Barry L Dowell

Mark Talmer

Wesley W Addison Ii

Silvia C Saldana

Ruan Qiaoqiao

The instant disclosure provides methods of multi-assay processing and multi-assay analysis. Such multi-assay processing and analysis pertain to automated detection of target nucleic acids, e.g., as performed in the clinical setting for diagnostic purposes. Also provided are common assay timing protocols derived from a variety of individual nucleic acid amplification and analysis protocols and modified to prevent resource contention. The instant disclosure also provides systems and devices for practicing the methods as described herein.

Methods and systems of multi-assay processing and analysis

Aspects of the present disclosure include sample analysis methods and systems. According to certain embodiments, provided are methods of analyzing samples in an automated sample analysis system. The methods include introducing samples and sample preparation cartridges into the system, isolating and purifying an analyte (e.g., nucleic acids and/or proteins) present in the samples at a sample preparation station, and performing analyte detection assays in assay mixtures that include the purified analyte. Also provided are automated sample analysis systems that find use, e.g., in performing the methods of the present disclosure. In certain aspects, the methods and systems provide for continuous operator access during replenishment or removal of one or any combination of samples, bulk fluids, reagents, commodities, waste, and/or the like.

Systems and methods for automated analysis

A method for analyzing the results of a ligand-receptor binding assay comprising the steps of: More particularly, the ligand-receptor binding assay involves the steps of combining appropriate reagents in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is directly proportional to the amount of ligand present in the sample. Alternatively, the ligand-receptor binding assay involves the steps of combining appropriate reagents to perform a ligand-receptor binding assay in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is inversely proportional to the amount of analyte present in the sample.

Spatially resolved ligand-receptor binding assays

Aspects of the present disclosure include sample preparation cartridges including a frame that includes a plurality of wells integrated therewith, where the plurality of wells have a closed bottom and an open top. The frame further includes an opening within the frame having a reaction vessel (RV) or RV cap removably disposed therein, where the plurality of wells and the opening are linearly arranged relative to each other. Also provided are sample preparation cartridges that include a frame, two or more cartridge separation projections on a top side of the frame, and two or more cartridge separation projections on a bottom side of the frame. The cartridge separation projections separate the cartridge and a different cartridge when the cartridge and different cartridge are stacked. Methods of using the sample preparation cartridges, as well as nucleic acid sample preparation units that include the sample preparation cartridges, are also provided.

Sample preparation cartridges and methods for using same

Provided herein are assays and kits useful for avoiding 'prozone phenomenon' or 'hook effect' and which expand the range of accurately measurable analyte concentrations.

Assay with increased dynamic range

A method for analyzing the results of a ligand-receptor binding assay comprising the steps of:

The invention relates to antibody characteristics used to design a whole blood Point of Care Thyroid Stimulating Hormone (TSH) immunoassay using an ELISA sandwich assay lacking one or more wash steps between the antigen capture, detection antibody addition and substrate introduction steps. This invention exhibits low cross reactivity with biologically similar interfering cross reacting species, such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).

TSH antibodies for point-of-care immunoassay formats

The invention relates to low wash Thyroid Stimulating Hormone (TSH) immunoassays using an ELISA sandwich assay having limited or no wash step between the antigen capture, detection antibody addition and substrate introduction steps. This invention exhibits low cross reactivity with biologically similar interfering cross reacting species, such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).

TSH immunoassays and processes for performing TSH immunoassays in the presence of endogenous contaminants in restricted wash formats

Growing community of inventors

Lake Villa, IL, United States of America

Joseph P Skinner