Life Technologies Corporation

Nanostring Technologies, Inc.

Bruker Spatial Biology, Inc.

University of Texas System

Invitrogen Corporation

Molecular Probes, Inc.

Joseph M Beechem

Dwayne L Dunaway

Rustem Khafizov

Dae Kim

Margaret Hoang

Mark Gregory

Theo Nikiforov

Xinzhan Peng

Sanghamithra Korukonda

Yi Deng

Elizabeth A Manrao

Richard P Haugland

Jaemyeong Jung

Michael Previte

Imad Naasani

Cheng-Yao Chen

David M Hagen

Joseph A Treadway

Dmitriy Gremyachinskiy

Daniel Mazur

Guobin Luo

Gordon B Mills

Eric Tulsky

Kari N Haley

Vi-En Choong

Roman Rozhkov

John Matthew Mauro

Yuri Belosludtsev

Howard Reese

Charles Warren

Chris Merritt

Scott Crowder

Kyle R Gee

Tommie Lloyd Lincecum

Robert Archer

Rosaria Haugland

Wayne Forrest Patton

Brian Agnew

Isaac Sprague

Thomas H Steinberg

Qian Mei

Lucas M Dennis

Michael Krouse

Erin Piazza

Kristina Sorg

Denise Zhou

Gavin Meredith

Mark McElwain

Peter Skene

Cassandra Burke

Jixiang Liu

Vladimir Martin

Iain Johnson

Matthew J Walsh

Gayathri Balasundaram

Matthew Walsh

Michael McKean

Hee-Chol Kang

Christina Pastula

Peter Askovich

Eliot Sosin

Grant Tremel

Tommie Lloyd Lincecum, Jr

Peter E Schultz

Bishop Wilkins

David C Hagen

Robert M Archer

Lucas Dennis

Shanshan He

Robert A Archer

Isabel Lee

Lidan Wu

The present invention relates to, among other things, probes, compositions, methods, and kits for simultaneous, multiplexed detection and quantification of protein expression in a user-defined region of a tissue, user-defined cell, and/or user-defined subcellular structure within a cell.

Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue

Systems, apparatuses and methods for spatially mapping at least one biological expression of a target biological component contained in a tissue sample to an image of the tissue sample are provided. In some embodiments, the system includes a processor and instructions that, when executed by the processor, cause the system to display, in a first display, a scans pane including at least the image of the tissue sample, the image including at least one demarcation corresponding to a region-of-interest (ROI(s)), where the ROI(s) correspond to a portion of the tissue within the tissue image. The instructions are further configured to cause the system to display, in a second display, a visualization pane including a visualization of the biological expression contained in the ROI(s); and to augment the first display by coding the ROI(s) in the tissue image to show the spatial mapping of the biological expression within the ROI(s).

Systems and methods for spatial mapping of expression profiling

The present disclosure relates to chemical compositions, kits, and apparatuses and methods for using these compositions, kits and apparatuses in various assays.

Chemical compositions and methods of using same

Embodiments of the present disclosure are directed to a compact, mobile, digital spatial profiling (DSP) system, and associated apparatuses, devices and methods, which are configured to image one or more regions-of-interest (ROIs), and then using UV light to cleave, for example, oligos off antibodies in one or more ROIs ('photocleaving'), and collect the photocleaved oligos for later hybridization and counting.

Methods, apparatuses, systems and devices for mobile digital spatial profiling of pathological specimens

The present invention relates to, among other things, probes, compositions, methods, and kits for simultaneous, multiplexed detection and quantification of protein and/or nucleic acid expression in a user-defined region of a tissue, user-defined cell, and/or user-defined subcellular structure within a cell.

Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue

The present invention relates to, among other things, probes, compositions, methods, and kits for simultaneous, multiplexed detection and quantification of protein and/or nucleic acid expression in a user-defined region of a tissue, user-defined cell, and/or user-defined subcellular structure within a cell that are adaptable for use with existing sequencing technologies.

Chemical compositions and uses thereof

The present disclosure is directed to systems, devices and methods for nucleic acid or protein purification and imaging. A system is provided including a cartridge comprising a sample input area configured to hold a sample, comprising a plurality of hybridized complexes comprising a plurality of target molecules each hybridized with probes and a plurality of non-hybridized probes. The cartridge may also include a first binding chamber configured with first magnetic beads to receive and bind the sample, a first elution channel configured to receive the first magnetic beads and elute the sample from the first magnetic beads, a second binding chamber configured with second magnetic beads to receive and bind the sample, a second elution channel configured to receive the second magnetic beads and elute the sample from the second magnetic beads, and a binding area configured to receive the eluted sample and hold molecules for imaging.

Methods and apparatuses for gene purification and imaging

Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Conjugates of biomolecules to nanoparticles

Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

Methods and apparatus for single molecule sequencing using energy transfer detection

Growing community of inventors

Eugene, OR, United States of America

Joseph M Beechem