Neogen Corporation

Perkinelmer Health Sciences, Inc.

Perkinelmer Las, Inc.

Perkinelmer Instruments LLC

Other

Joseph L DiCesare

David M Aikens

Timothy P Neal

John T McCaffrey

David J Clark

Michael I Crockett

Gregory A Hanlon

Richard Edwards

Feng Jin

Gitesh Kumar

Gregory Hanlon

A flow cell for a fluorescence spectrometer includes a flow channel to receive a flow of a liquid sample, an excitation light entrance window to receive excitation light from a light source, and an emission light exit window to transmit fluorescent emission light from the liquid sample in the flow channel from the flow cell. The excitation light entrance window and/or emission light exit window includes a waveguide including: a waveguide core formed of a core material; and a cladding medium surrounding a portion of the waveguide core, wherein the cladding medium has a refractive index less than the refractive index of the core material. The waveguide defines a portion of the flow channel.

Flow cell modules and liquid sample analyzers and methods including same

A liquid sample analyzer includes a liquid sample source, a flow cell, an optical device and a plurality of optical fibers. The flow cell is configured to receive a flow of a liquid sample from the liquid sample source. The plurality of optical fibers optically connect the flow cell to the optical device to transmit light between the flow cell and the optical device.

A flow cell assembly for use in a liquid sample analyzer including a radiation source, a sensing device and a liquid sample source to supply a liquid sample includes an entrance joint member, a liquid core waveguide, a liquid sample feed tube, and an input optical fiber. The entrance joint member includes a waveguide receiving bore and a feed tube receiving bore. The liquid core waveguide is mounted in the waveguide receiving bore and defines a waveguide bore. The liquid sample feed tube is mounted in the feed tube receiving bore such that the liquid sample feed tube is in fluid communication with the waveguide bore to fluidly connect the liquid sample source to the waveguide bore. The input optical fiber is mounted in the entrance joint member to transmit radiation from the radiation source to the waveguide bore, which radiation is transmitted through the waveguide bore and the liquid sample therein to the sensing device.

Flow cell assembly for liquid sample analyzer

A method of scanning a sample plate surface mask in an area adjacent to a conductive area using mass spectrometry is disclosed. The method comprises the steps of providing a sample plate including a mask applied with a rough surface to the electrically conductive surface to produce a sample site comprising a central portion formed from the electrically conductive surface and a marginal portion of the mask, preparing an analyte comprising mixing a biomolecule with an organic solvent, an aqueous solution, and a matrix selected from the group of &#x3b1;-cyano-4-hydroxycinnamic acid and3,5-dimethoxy-4-hydroxycinnamic acid; applying the analyte to the sample site; forming at least one crystal of the analyte in an area on the mask adjacent to the conductive area, and scanning the area on the mask adjacent to the conductive area with a laser beam.

Method of scanning a sample plate surface mask in an area adjacent to a conductive area using matrix-assisted laser desorption and ionization mass spectrometry

Disclosed herein is a device and methods for the rapid chemiluminescence assay of surfaces to detect the presence of microbial contamination. The device and methods are suitable for use by untrained personnel under the relatively harsh and variable conditions found in the field, for example in fast food restaurants and other food preparation areas. The chemiluminescence reaction that is the source of the analytical signal in the disclosed assay device and method is preferably based on a luciferase/luciferin system. The method for sampling disclosed herein comprises the steps of pre-wetting the sampling swab to a level below that of absorptive saturation; wiping a surface to be sampled with the swab with sufficient pressure to expel the wetting solution onto the surface; and, after reducing the pressure exerted on the sampling swab, further wiping the surface to re-absorb the moisture from the surface.

Apparatus for chemiluminescent assays

High density multiplexing assays for proteins, nucleic acids, and other molecules are described based upon using mass spectrometry detection. A solid-state support assay format is utilized, whereby, the bead carries both a coding molecule to track the sample or analyte and the molecule(s) necessary to perform an affinity-based assay. After the necessary reactions are complete, all of the components, e.g., coding molecule, binding molecule, analyte, and/or label are dissociated for analysis in a mass spectrometer. Various means of performing detection for the assays include different types of mass spectrometers with different types of sampling systems, including MALDI-TOF and ESI.

Multiplexing assays for analyte detection

A method of making a sample plate for mass spectrometric analysis of a specimen is provided comprising applying a mask to a substrate to form at least one sample site. The sample plate comprises a substrate having an electrically conductive surface, a mask applied to the electrically conductive surface. The mask has a rougher surface than the substrate. The sample site comprises a central portion formed from the electrically conductive surface and a marginal portion formed from the mask where the marginal portion is more hydrophobic than the central portion.

Sample plate for matrix-assisted laser desorption and ionization mass spectrometry

Disclosed herein is a polymeric sampling swab for obtaining samples of an analyte of interest from solid surfaces or from liquid substances. The polymeric material of which the swab is composed is characterized by a high resistance to chemical and mechanical degradation. The sampling swab of the present invention is further characterized by a high internal void volume and a high absorptive capacity for fluids. The swab of the present invention is particularly suited for obtaining samples for use in chemiluminescent assays for, among other analytes of interest, microbial contamination. Also disclosed is a polymeric disc for loading with reagent mixtures suitable for use in bioluminescent assay procedures. The reagent disc of the invention is characterized by high resistance to chemical and mechanical degradation. In addition, the disc has a high void volume and high absorptive capacity.

Polymeric medium for the retention of reagent species for use in a hand-held device for the relatively rapid detection of the presence of an analyte of interest in a sample

A sample plate for mass spectrometric analysis of a specimen is provided comprising a substrate having an electrically conductive surface, a mask applied to the electrically conductive surface. The mask is applied to the substrate with a rough surface to form at least one sample site. The sample site comprises a central portion formed from the electrically conductive surface and a marginal portion formed from the mask where the marginal portion is more hydrophobic than the central portion.

Disclosed is a device and methods for the rapid chemiluminescence assay of surfaces to detect the presence of microbial contamination. The device and methods are suitable for use by untrained personnel under the relatively harsh and variable conditions found in the field, for example in fast food restaurants and other food preparation areas. The chemiluminescence reaction that is the source of the analytical signal in the disclosed assay device and method is preferably based on a luciferase/luciferin system.

Apparatus and methods for chemiluminescent assays

Disclosed herein are methods for the chemiluminescent assay of a variety of analytes of interest. The methods are adaptable to the determination of microbial species in both liquid samples and on solid surfaces. The disclosed methods can be used with rapid, self-contained chemiluminescence assay devices, or can be used with novel sampling devices and conventional microbial analysis techniques involving growth of microbial samples on appropriate culturing media. The specificity of the methods can be enhanced with the use of immunospecific reagents. The sensitivity of the technique can be increased by 3 to 6 orders of magnitude by first converting all DNA in the sample to inorganic phosphates before generating the emission signal. The breadth of applicability of the disclosed methods can be enhanced through the selection of appropriate enzyme-catalyzed reactions where one of the products of enzymatic oxidation is hydrogen peroxide.

Growing community of inventors

Redding, CT, United States of America

Joseph L DiCesare