Dow Agrosciences LLC

Sangamo Therapeutics, Inc.

Sangamo Biosciences, Inc.

Corteva Agriscience LLC

Phyton Holdings, LLC

Joseph F Petolino

Jeffrey C Miller

Steven Robert Webb

Ryan C Blue

Qihua C Cai

Dayakar Reddy Pareddy

Robbi J Garrison

Fyodor Urnov

W Michael Ainley

Steven Lee Evans

Nicole Linda Hopkins

Manju Gupta

Sarah E Worden

Stephen N Novak

Jayakumar Pon Samuel

Nicole Arnold

Russell DeKelver

Vipula Shukla

Jon Christopher Mitchell

Katherine Armstrong

Beth C Rubin-Wilson

Jianquan Li

Lisa W Baker

German Carlos Spangenberg

Aaron Todd Woosley

Lakshmi Sastry-Dent

Otto Folkerts

Dmitry Y Guschin

William M Ainley

Andrew F Worden

Kelley Ann Smith

Kerrm Y Yau

Narasimha Chary Samboju

Matthew Hayden

Tim Sawbridge

Yidong Ran

John Mason

Lisa L Schulenberg

Michael A Menke

Scott Belmar

Michael Ainley

Daniel Isenegger

David Richard Corbin

James Wayne Bing

Andrew C Hiatt

Ning Zhou

Gregory J Cost

Mich B Hein

Sean Michael Russell

W H Kerr Anderson

Steven Arnold Thompson

Jeffrey A Miller

Jeffrey Beringer

Kristen Briggs

Weiting W Ni

Jean L Roberts

Raghav Ram

Asha Mehra Palta

Mary E Welter

Todd Glancy

Anton S Karnoup

Doug Taylor

Pon Samuel Jayakumar

Bridget D Kosegi

Russell Dekelver

German Spangenberg

Beth Cori Rubin-Wilson

Robbi Janette Garrison

William Michael Ainley

Andrew Frederick Worden

Lisa Lynn Schulenberg

Steven L Evans

Jeffrey Miller

Vipula K Shukla

Nicole L Arnold

This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

Plant transactivation interaction motifs and uses thereof

Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

Methods and compositions for integration of an exogenous sequence within the genome of plants

Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into a predetermined target site in a plant genome.

Zinc finger nuclease-mediated homologous recombination

Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.

Optimized non-canonical zinc finger proteins

The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Precision gene targeting to a particular locus in maize

Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

Methods and compositions for nuclease-mediated targeted integration of transgenes

The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis

Provided are methods for introducing a sequence-specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Nanoparticle mediated delivery of sequence specific nucleases

A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

Excision of transgenes in genetically modified organisms

The present invention relates to methods and compositions for the expression of protein. Embodiments of methods may comprise the cleavage and repair of a nucleotide sequence encoding a highly expressed protein leading to a reduction in the expression of the highly expressed protein.

Protein production in plant cells and associated methods and compositions

Growing community of inventors

Zionsville, IN, United States of America

Joseph F Petolino