Monsanto Technology LLC

Joseph E Huesing

Charles P Romano

Scott E Andersen

Clayton S Vetsch

Glenn R Hicks

Gregory Hinkle

David Richard Corbin

Thomas M Malvar

Barry S Goldman

Steven C Slater

Karina Krasomil-Osterfeld

Sergei Spiridonov

Expressed Sequence Tags (ESTs) isolated from the Western Corn Rootworm,LeConte, are disclosed. The invention encompasses nucleic acid molecules that encodeprotein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.

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The present invention relates to a nucleic acid sequences fromthat encodes a GroEL protein. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins, fragments of proteins, tRNA's, fragments of tRNA's, rRNA's and fragments of rRNA's. In addition, protein and fragments of proteins so encoded and antibodies capable of binding the proteins are encompassed by the present invention. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, RNA'S, and antibodies, for example, for gene identification and analysis, and preparation of constructs.

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Chesterfield, MO, United States of America

Joseph E Huesing